Detection of Biologically Relevant Anions by Fluorescence and NIR Molecular Probes

This thesis documented our research efforts aimed at the detection of biologically relevant anions using molecular probes. The first part has demonstrated that hydroethidine is oxidized to ethidium under the pro-oxidative effect of flavonols under physiological conditions and is a sensitive fluorescent probe for rapid quantification of the superoxide radical-forming pro-oxidant activity of flavonols. This assay was then successfully applied in quantifying the pro-oxidant activity of different tea leaves. The second part has demonstrated that a near infrared (NIR) active mixed-valence diruthenium complex can catalyze the air oxidation of HS- to generate hydrogen peroxide, disulfane, and elemental sulfur. The NIR probe provides a convenient way for the detection of the HS- generation rate of a H2S donor of medical importance. The last part describes the synthesis and characterization of two novel NIR active diiron complexes. Their reactivity towards various redox-active molecules was evaluated.

Author dc:creator

• QUEK YI LING

• pro-oxidant, superoxide, hydroethidine, fluorescent probe, bimetallic complex, NIR probe