{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/318722"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/318722","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"DIABETES MELLITUS AND THE DYSREGULATION OF ANGIOGENESIS IN TUBERCULOSIS","abstract":"Diabetes mellitus (DM), characterized by chronic hyperglycemia, is a risk factor for tuberculosis (TB), contributing to more severe disease and poorer treatment outcomes. Angiogenesis plays a key role in TB immunopathology by supporting mycobacterial survival within granulomas, whereas DM-associated oxidative stress impairs normal angiogenic processes. I hypothesized that disrupted angiogenic regulation underlies the worsened outcomes observed in DM-TB comorbidity. To investigate this, I profiled angiogenic factors and matrix metalloproteinases in PBMCs and neutrophils stimulated with heat-killed Mycobacterium tuberculosis, alongside transcriptional analysis using single-cell RNA sequencing. Infection conditions were optimized based on reactive oxygen species production and cell viability. Following stimulation, a multiplex Luminex assay revealed early induction of angiogenic factors in both healthy and DM groups, with a trend toward reduced secretion in DM. scRNA-seq identified angiogenic gene signatures in select myeloid populations. In summary, this study provides insight into the early landscape of angiogenic signalling in DM-TB.","abstract_html":"Diabetes mellitus (DM), characterized by chronic hyperglycemia, is a risk factor for tuberculosis (TB), contributing to more severe disease and poorer treatment outcomes. Angiogenesis plays a key role in TB immunopathology by supporting mycobacterial survival within granulomas, whereas DM-associated oxidative stress impairs normal angiogenic processes. I hypothesized that disrupted angiogenic regulation underlies the worsened outcomes observed in DM-TB comorbidity. To investigate this, I profiled angiogenic factors and matrix metalloproteinases in PBMCs and neutrophils stimulated with heat-killed Mycobacterium tuberculosis, alongside transcriptional analysis using single-cell RNA sequencing. Infection conditions were optimized based on reactive oxygen species production and cell viability. Following stimulation, a multiplex Luminex assay revealed early induction of angiogenic factors in both healthy and DM groups, with a trend toward reduced secretion in DM. scRNA-seq identified angiogenic gene signatures in select myeloid populations. In summary, this study provides insight into the early landscape of angiogenic signalling in DM-TB.","abstract_has_math":false,"creators":["CHONG HAI TARNG"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-08-20","date_published":"2025-08-20","updated_at":"2026-07-24T03:32:18Z","subjects":["immune response","Host-pathogen interaction","scRNA-seq","Angiogenesis","Diabetes Mellitus","Tuberculosis"],"languages":[],"rights":[],"rights_urls":["https://scholarbank.nus.edu.sg/bitstreams/2a93199f-4703-4afb-87ab-c640e6bd5b3f/download"],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["CHONG HAI TARNG"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2025-08-20"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/318722"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["immune response","Host-pathogen interaction","scRNA-seq","Angiogenesis","Diabetes Mellitus","Tuberculosis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["https://scholarbank.nus.edu.sg/bitstreams/2a93199f-4703-4afb-87ab-c640e6bd5b3f/download"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/4d4a01c5-1f6c-4698-bb8f-7fc2862eb29d/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Diabetes mellitus (DM), characterized by chronic hyperglycemia, is a risk factor for tuberculosis (TB), contributing to more severe disease and poorer treatment outcomes. Angiogenesis plays a key role in TB immunopathology by supporting mycobacterial survival within granulomas, whereas DM-associated oxidative stress impairs normal angiogenic processes. I hypothesized that disrupted angiogenic regulation underlies the worsened outcomes observed in DM-TB comorbidity. To investigate this, I profiled angiogenic factors and matrix metalloproteinases in PBMCs and neutrophils stimulated with heat-killed Mycobacterium tuberculosis, alongside transcriptional analysis using single-cell RNA sequencing. Infection conditions were optimized based on reactive oxygen species production and cell viability. Following stimulation, a multiplex Luminex assay revealed early induction of angiogenic factors in both healthy and DM groups, with a trend toward reduced secretion in DM. scRNA-seq identified angiogenic gene signatures in select myeloid populations. In summary, this study provides insight into the early landscape of angiogenic signalling in DM-TB."]},{"key":"dc:format.checksum.md5","label":"Dc Format Checksum Md5","values":["9f3c6f2aa8f96291ef77b0a18484521d","7ec0f29bfdc864e49394005f3fb9558c","c4b5c725d7937eb38867926f4c641b9e"]},{"key":"dc:title","label":"Title","values":["DIABETES MELLITUS AND THE DYSREGULATION OF ANGIOGENESIS IN TUBERCULOSIS"]}]}],"canonical_facts":{"dc:creator":["CHONG HAI TARNG"],"dc:date.issued":["2025-08-20"],"dc:description.abstract":["Diabetes mellitus (DM), characterized by chronic hyperglycemia, is a risk factor for tuberculosis (TB), contributing to more severe disease and poorer treatment outcomes. Angiogenesis plays a key role in TB immunopathology by supporting mycobacterial survival within granulomas, whereas DM-associated oxidative stress impairs normal angiogenic processes. I hypothesized that disrupted angiogenic regulation underlies the worsened outcomes observed in DM-TB comorbidity. To investigate this, I profiled angiogenic factors and matrix metalloproteinases in PBMCs and neutrophils stimulated with heat-killed Mycobacterium tuberculosis, alongside transcriptional analysis using single-cell RNA sequencing. Infection conditions were optimized based on reactive oxygen species production and cell viability. Following stimulation, a multiplex Luminex assay revealed early induction of angiogenic factors in both healthy and DM groups, with a trend toward reduced secretion in DM. scRNA-seq identified angiogenic gene signatures in select myeloid populations. In summary, this study provides insight into the early landscape of angiogenic signalling in DM-TB."],"dc:format.checksum.md5":["9f3c6f2aa8f96291ef77b0a18484521d","7ec0f29bfdc864e49394005f3fb9558c","c4b5c725d7937eb38867926f4c641b9e"],"dc:identifier.uri":["https://scholarbank.nus.edu.sg/bitstreams/4d4a01c5-1f6c-4698-bb8f-7fc2862eb29d/download"],"dc:relation.isreferencedby":["https://scholarbank.nus.edu.sg/handle/10635/318722"],"dc:rights":["https://scholarbank.nus.edu.sg/bitstreams/2a93199f-4703-4afb-87ab-c640e6bd5b3f/download"],"dc:subject":["immune response","Host-pathogen interaction","scRNA-seq","Angiogenesis","Diabetes Mellitus","Tuberculosis"],"dc:title":["DIABETES MELLITUS AND THE DYSREGULATION OF ANGIOGENESIS IN TUBERCULOSIS"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:32:18Z"}