STUDYING THE INTERACTION OF NANO- & MICROPLASTICS WITH ENTEROVIRUS AND THEIR CYTOTOXICITY ON HUMAN LUNG CELL LINE
type: CreativeWork
description: Uncontrolled plastic waste degrades into micro- and nanoplastic particles that can be ingested by living organisms, including humans. These particles are of concern due to their ability to adsorb contaminants and their potential long-term cellular effects. This study investigated the interaction between enterovirus D68 (EV-D68) and charged nano- and microplastics, as well as the chronic effects of positively charged mixed polymer nanoplastic particles on a human lung cell line. Positively charged poly(methyl methacrylate) (PMMA) surfaces exhibited enhanced binding to EV-D68 compared with neutral and negatively charged surfaces, resulting in significant viral reduction. PMMA fibres containing 5 mg cetyltrimethylammonium bromide (CTAB) achieved the greatest reduction but retained infectivity, suggesting a possible role as viral vectors. Long-term exposure studies demonstrated rapid nanoplastic internalisation and inheritance across 11 generations. Elevated lactate dehydrogenase levels and increased apoptosis indicated persistent intracellular accumulation and compromised cellular health.
abstract: Uncontrolled plastic waste degrades into micro- and nanoplastic particles that can be ingested by living organisms, including humans. These particles are of concern due to their ability to adsorb contaminants and their potential long-term cellular effects. This study investigated the interaction between enterovirus D68 (EV-D68) and charged nano- and microplastics, as well as the chronic effects of positively charged mixed polymer nanoplastic particles on a human lung cell line. Positively charged poly(methyl methacrylate) (PMMA) surfaces exhibited enhanced binding to EV-D68 compared with neutral and negatively charged surfaces, resulting in significant viral reduction. PMMA fibres containing 5 mg cetyltrimethylammonium bromide (CTAB) achieved the greatest reduction but retained infectivity, suggesting a possible role as viral vectors. Long-term exposure studies demonstrated rapid nanoplastic internalisation and inheritance across 11 generations. Elevated lactate dehydrogenase levels and increased apoptosis indicated persistent intracellular accumulation and compromised cellular health.
author: KRITIKA PANDEY
keywords: Transgenerational nanoplastic inheritance, Nanotoxicology, H1299 Cells, EV-D68, Viral-polymer interactions, Nanoplastics, Microplastics
license: https://scholarbank.nus.edu.sg/bitstreams/e01a96ea-11f4-4ae2-8322-3542110f8887/download
date published: 2025-07-11
date modified: 2026-07-24T03:32:43Z
provider: National University of Singapore
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: National University of Singapore