{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/317978"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/317978","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"STUDYING THE INTERACTION OF NANO- & MICROPLASTICS WITH ENTEROVIRUS AND THEIR CYTOTOXICITY ON HUMAN LUNG CELL LINE","abstract":"Uncontrolled plastic waste degrades into micro- and nanoplastic particles that can be ingested by living organisms, including humans. These particles are of concern due to their ability to adsorb contaminants and their potential long-term cellular effects. This study investigated the interaction between enterovirus D68 (EV-D68) and charged nano- and microplastics, as well as the chronic effects of positively charged mixed polymer nanoplastic particles on a human lung cell line. Positively charged poly(methyl methacrylate) (PMMA) surfaces exhibited enhanced binding to EV-D68 compared with neutral and negatively charged surfaces, resulting in significant viral reduction. PMMA fibres containing 5 mg cetyltrimethylammonium bromide (CTAB) achieved the greatest reduction but retained infectivity, suggesting a possible role as viral vectors. Long-term exposure studies demonstrated rapid nanoplastic internalisation and inheritance across 11 generations. Elevated lactate dehydrogenase levels and increased apoptosis indicated persistent intracellular accumulation and compromised cellular health.","abstract_html":"Uncontrolled plastic waste degrades into micro- and nanoplastic particles that can be ingested by living organisms, including humans. These particles are of concern due to their ability to adsorb contaminants and their potential long-term cellular effects. This study investigated the interaction between enterovirus D68 (EV-D68) and charged nano- and microplastics, as well as the chronic effects of positively charged mixed polymer nanoplastic particles on a human lung cell line. Positively charged poly(methyl methacrylate) (PMMA) surfaces exhibited enhanced binding to EV-D68 compared with neutral and negatively charged surfaces, resulting in significant viral reduction. PMMA fibres containing 5 mg cetyltrimethylammonium bromide (CTAB) achieved the greatest reduction but retained infectivity, suggesting a possible role as viral vectors. Long-term exposure studies demonstrated rapid nanoplastic internalisation and inheritance across 11 generations. Elevated lactate dehydrogenase levels and increased apoptosis indicated persistent intracellular accumulation and compromised cellular health.","abstract_has_math":false,"creators":["KRITIKA PANDEY"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-07-11","date_published":"2025-07-11","updated_at":"2026-07-24T03:32:43Z","subjects":["Transgenerational nanoplastic inheritance","Nanotoxicology","H1299 Cells","EV-D68","Viral-polymer interactions","Nanoplastics","Microplastics"],"languages":[],"rights":[],"rights_urls":["https://scholarbank.nus.edu.sg/bitstreams/e01a96ea-11f4-4ae2-8322-3542110f8887/download"],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["KRITIKA PANDEY"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2025-07-11"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/317978"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Transgenerational nanoplastic inheritance","Nanotoxicology","H1299 Cells","EV-D68","Viral-polymer interactions","Nanoplastics","Microplastics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["https://scholarbank.nus.edu.sg/bitstreams/e01a96ea-11f4-4ae2-8322-3542110f8887/download"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/aeafa158-bdcf-4043-b04c-7f4c7cfa8eae/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Uncontrolled plastic waste degrades into micro- and nanoplastic particles that can be ingested by living organisms, including humans. These particles are of concern due to their ability to adsorb contaminants and their potential long-term cellular effects. This study investigated the interaction between enterovirus D68 (EV-D68) and charged nano- and microplastics, as well as the chronic effects of positively charged mixed polymer nanoplastic particles on a human lung cell line. Positively charged poly(methyl methacrylate) (PMMA) surfaces exhibited enhanced binding to EV-D68 compared with neutral and negatively charged surfaces, resulting in significant viral reduction. PMMA fibres containing 5 mg cetyltrimethylammonium bromide (CTAB) achieved the greatest reduction but retained infectivity, suggesting a possible role as viral vectors. Long-term exposure studies demonstrated rapid nanoplastic internalisation and inheritance across 11 generations. 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This study investigated the interaction between enterovirus D68 (EV-D68) and charged nano- and microplastics, as well as the chronic effects of positively charged mixed polymer nanoplastic particles on a human lung cell line. Positively charged poly(methyl methacrylate) (PMMA) surfaces exhibited enhanced binding to EV-D68 compared with neutral and negatively charged surfaces, resulting in significant viral reduction. PMMA fibres containing 5 mg cetyltrimethylammonium bromide (CTAB) achieved the greatest reduction but retained infectivity, suggesting a possible role as viral vectors. Long-term exposure studies demonstrated rapid nanoplastic internalisation and inheritance across 11 generations. 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