Functional analysis of syp1, a novel substrate of the serine/threonine kinase prk1
type: CreativeWork
description: In budding yeast, the actin and septin cytoskeletons are important for the establishment of cell polarity. During the cell cycle, these cytoskeletons undergo dramatic reorganization which is regulated by many cytoskeleton-associated proteins. In this study, Syp1p has been found to have functional interactions with the actin cytoskeleton. Syp1p is a new substrate of Prk1p, a kinase that regulates actin dynamics. Syp1p colocalizes with actin cytoskeleton whose integrity is required for the polarized localization of Syp1p. Syp1p can physically interact with the actin-associated protein Sla1p. Furthermore, Syp1p overexpression suppresses the defects of BNI1 deletion mutant. In addition to its roles in actin cytoskeleton, Syp1p is also discovered to be a new regulator of the septin dynamics. Syp1p colocalizes and physically interacts with septins. Syp1p overexpression disorganizes septin structure and induces the Swe1p-dependent elongated bud phenotype. Syp1p also regulates the organization of septins in different cell cycle stages.
abstract: In budding yeast, the actin and septin cytoskeletons are important for the establishment of cell polarity. During the cell cycle, these cytoskeletons undergo dramatic reorganization which is regulated by many cytoskeleton-associated proteins. In this study, Syp1p has been found to have functional interactions with the actin cytoskeleton. Syp1p is a new substrate of Prk1p, a kinase that regulates actin dynamics. Syp1p colocalizes with actin cytoskeleton whose integrity is required for the polarized localization of Syp1p. Syp1p can physically interact with the actin-associated protein Sla1p. Furthermore, Syp1p overexpression suppresses the defects of BNI1 deletion mutant. In addition to its roles in actin cytoskeleton, Syp1p is also discovered to be a new regulator of the septin dynamics. Syp1p colocalizes and physically interacts with septins. Syp1p overexpression disorganizes septin structure and induces the Swe1p-dependent elongated bud phenotype. Syp1p also regulates the organization of septins in different cell cycle stages.
author: QIU WENJIE
keywords: actin, septin, Syp1p, Prk1p, cell division, cytokinesis
date published: 2007-10-30
date modified: 2026-07-24T03:30:47Z
provider: National University of Singapore
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: National University of Singapore