{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/209023"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/209023","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"DEVELOPMENT OF NON-CONTACT MICROSPHERE CONFOCAL SCANNING MICROSCOPE","abstract":"The confocal scanning microscope has proven to be a powerful tool to produce high-quality three-dimensional (3D) imaging. However, the resolution of the confocal laser scanning microscope in the visible spectrum is restricted to be ~180 nm and ~500 nm in the lateral and axial direction respectively due to the optical diffraction. In this thesis, a novel technique, called the non-contact microsphere confocal scanning microscope, is proposed and developed. 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This setup can enhance the lateral resolution to 110 nm, the axial resolution to 100nm and image contrast to 71%, while retaining the key features of the conventional confocal microscope, such as no damage to samples, 3D in-vivo imaging and the ability to work in different environments."]},{"key":"dc:format.checksum.md5","label":"Dc Format Checksum Md5","values":["eeac6b91ee6719a61512135227709e83","3d914d9cde66581eff641b0235519133"]},{"key":"dc:title","label":"Title","values":["DEVELOPMENT OF NON-CONTACT MICROSPHERE CONFOCAL SCANNING MICROSCOPE"]}]}],"canonical_facts":{"dc:creator":["YANG XILIANG"],"dc:date.issued":["2021-07-31"],"dc:description.abstract":["The confocal scanning microscope has proven to be a powerful tool to produce high-quality three-dimensional (3D) imaging. However, the resolution of the confocal laser scanning microscope in the visible spectrum is restricted to be ~180 nm and ~500 nm in the lateral and axial direction respectively due to the optical diffraction. 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