SELECTION OF APTAMERS FOR INTESTINAL PERMEABILITY TEST.

Since its first description in 1990, SELEX (Systematic Evolution of Ligands by Exponential enrichment) technology has been widely used as an in vitro selection method to evolve nucleic acid ligands, called aptamers, with new functionalities. We have prepared single-stranded DNA aptamers against non-metabolizable sugars and peptide using SELEX, with the ultimate goal of developing biosensors for the intestinal permeability test. Design strategy for such aptamer-based biosensors is also proposed.

Author dc:creator

• LI GUOLIANG

• aptamers, SELEX (Systematic Evolution of Ligands by Exponential enrichment), Intestinal permeability test, biosensor