{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/191691"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/191691","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"INVESTIGATING THE ROLE OF SPECIALIZED RIBOSOME DURING ERYTHROPOIESIS","abstract":"Eukaryotic ribosomes are made up of ~80 ribosomal proteins (RPs) and 4 ribosomal RNAs (rRNAs), and are traditionally thought to be invariant in all cells. Recent studies have found that ribosomes can be heterogeneous in different cell types, and can regulate translation of specific subsets of mRNAs. Through proteomics study of polysomes in differentiating erythroblasts, we show that the pool of ribosomes changes during erythropoiesis. Selective ribosome profiling show that certain subsets of mRNAs, including those important in erythropoiesis, are preferentially bound to ribosomes containing these RPs. These mRNAs exhibit weaker translation initiation contexts, when compared to a background. Even so, translation efficiencies of these mRNAs show concomitant increases with expression of the specialized ribosomes. Our study also outlines a novel post-translational RP alteration on the mature ribosome, uncovering a new mechanism to form heterogeneous ribosomes. Together, our study reveals important regulatory functions of specialized ribosomes in driving proper erythropoiesis.","abstract_html":"Eukaryotic ribosomes are made up of ~80 ribosomal proteins (RPs) and 4 ribosomal RNAs (rRNAs), and are traditionally thought to be invariant in all cells. Recent studies have found that ribosomes can be heterogeneous in different cell types, and can regulate translation of specific subsets of mRNAs. Through proteomics study of polysomes in differentiating erythroblasts, we show that the pool of ribosomes changes during erythropoiesis. Selective ribosome profiling show that certain subsets of mRNAs, including those important in erythropoiesis, are preferentially bound to ribosomes containing these RPs. These mRNAs exhibit weaker translation initiation contexts, when compared to a background. Even so, translation efficiencies of these mRNAs show concomitant increases with expression of the specialized ribosomes. Our study also outlines a novel post-translational RP alteration on the mature ribosome, uncovering a new mechanism to form heterogeneous ribosomes. Together, our study reveals important regulatory functions of specialized ribosomes in driving proper erythropoiesis.","abstract_has_math":false,"creators":["NAH JIE MIN"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-11-30","date_published":"2020-11-30","updated_at":"2026-07-24T03:33:34Z","subjects":["TRANSLATION, RIBOSOMES, RNA, ERYTHROPOIESIS"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["NAH JIE MIN"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2020-11-30"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/191691"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["TRANSLATION, RIBOSOMES, RNA, ERYTHROPOIESIS"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/d9770b62-3cf2-417e-a08b-5d1430878b78/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Eukaryotic ribosomes are made up of ~80 ribosomal proteins (RPs) and 4 ribosomal RNAs (rRNAs), and are traditionally thought to be invariant in all cells. Recent studies have found that ribosomes can be heterogeneous in different cell types, and can regulate translation of specific subsets of mRNAs. Through proteomics study of polysomes in differentiating erythroblasts, we show that the pool of ribosomes changes during erythropoiesis. Selective ribosome profiling show that certain subsets of mRNAs, including those important in erythropoiesis, are preferentially bound to ribosomes containing these RPs. These mRNAs exhibit weaker translation initiation contexts, when compared to a background. Even so, translation efficiencies of these mRNAs show concomitant increases with expression of the specialized ribosomes. Our study also outlines a novel post-translational RP alteration on the mature ribosome, uncovering a new mechanism to form heterogeneous ribosomes. Together, our study reveals important regulatory functions of specialized ribosomes in driving proper erythropoiesis."]},{"key":"dc:format.checksum.md5","label":"Dc Format Checksum Md5","values":["a493de55df9fdbffe7674cefbff60acf","db77b7c2988109bd319c2094d917ffa3"]},{"key":"dc:title","label":"Title","values":["INVESTIGATING THE ROLE OF SPECIALIZED RIBOSOME DURING ERYTHROPOIESIS"]}]}],"canonical_facts":{"dc:creator":["NAH JIE MIN"],"dc:date.issued":["2020-11-30"],"dc:description.abstract":["Eukaryotic ribosomes are made up of ~80 ribosomal proteins (RPs) and 4 ribosomal RNAs (rRNAs), and are traditionally thought to be invariant in all cells. Recent studies have found that ribosomes can be heterogeneous in different cell types, and can regulate translation of specific subsets of mRNAs. Through proteomics study of polysomes in differentiating erythroblasts, we show that the pool of ribosomes changes during erythropoiesis. Selective ribosome profiling show that certain subsets of mRNAs, including those important in erythropoiesis, are preferentially bound to ribosomes containing these RPs. These mRNAs exhibit weaker translation initiation contexts, when compared to a background. Even so, translation efficiencies of these mRNAs show concomitant increases with expression of the specialized ribosomes. Our study also outlines a novel post-translational RP alteration on the mature ribosome, uncovering a new mechanism to form heterogeneous ribosomes. 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