{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/179556"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/179556","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"PRODUCTION OF VIRUS-FREE ORCHIDS VIA TISSUE CULTURE TECHNIQUES","abstract":"Due to constant handling and vegetative propagation, most commercially cultivated orchid hybrids are systemically infected with cymbidium mosaic virus (CyMV) and/or odontoglossum ringspot virus (ORSV). Two tissue culture techniques were employed in this project to eliminate the viral pathogens from the infected orchid plants: meristem culture and thin section culture with/without chemotherapy. Meristems (0.10 mm to 1.00 mm) were excised from axillary shoots of CyMV and ORSV infected Mokara Char Kuan 'Pink', a monopodial hybrid and CyMV-infected Cattleya Bowbells, a sympodial hybrid. Although the survival rate was very low, two plants were obtained from the larger meristems. However, these plants remained virus-infected. In contrast, thin section culture of both shoot and protocorm-like bodies yielded many virus-free plants regardless of the presence of ribavirin. A highly sensitive diagnostic test for CyMV was also developed based on the polymerase chain reaction. As little as 1 fg of viral RNA (about 200 target copies) or 10 fg of purified virus (approximately 130 viral particles) could be detected. Only 1 mm3 of plant sample was required and the results could be obtained in 5 h.","abstract_html":"Due to constant handling and vegetative propagation, most commercially cultivated orchid hybrids are systemically infected with cymbidium mosaic virus (CyMV) and/or odontoglossum ringspot virus (ORSV). Two tissue culture techniques were employed in this project to eliminate the viral pathogens from the infected orchid plants: meristem culture and thin section culture with/without chemotherapy. Meristems (0.10 mm to 1.00 mm) were excised from axillary shoots of CyMV and ORSV infected Mokara Char Kuan 'Pink', a monopodial hybrid and CyMV-infected Cattleya Bowbells, a sympodial hybrid. Although the survival rate was very low, two plants were obtained from the larger meristems. However, these plants remained virus-infected. In contrast, thin section culture of both shoot and protocorm-like bodies yielded many virus-free plants regardless of the presence of ribavirin. A highly sensitive diagnostic test for CyMV was also developed based on the polymerase chain reaction. As little as 1 fg of viral RNA (about 200 target copies) or 10 fg of purified virus (approximately 130 viral particles) could be detected. Only 1 mm3 of plant sample was required and the results could be obtained in 5 h.","abstract_has_math":false,"creators":["LIM SIEW TIN"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1993,"date_issued":"1993","date_published":"1993","updated_at":"2026-07-24T03:30:47Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["LIM SIEW TIN"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["1993"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/179556"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/1048bf4d-955a-4fdd-b6f9-e51a94cfbe48/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Due to constant handling and vegetative propagation, most commercially cultivated orchid hybrids are systemically infected with cymbidium mosaic virus (CyMV) and/or odontoglossum ringspot virus (ORSV). Two tissue culture techniques were employed in this project to eliminate the viral pathogens from the infected orchid plants: meristem culture and thin section culture with/without chemotherapy. Meristems (0.10 mm to 1.00 mm) were excised from axillary shoots of CyMV and ORSV infected Mokara Char Kuan 'Pink', a monopodial hybrid and CyMV-infected Cattleya Bowbells, a sympodial hybrid. Although the survival rate was very low, two plants were obtained from the larger meristems. However, these plants remained virus-infected. In contrast, thin section culture of both shoot and protocorm-like bodies yielded many virus-free plants regardless of the presence of ribavirin. A highly sensitive diagnostic test for CyMV was also developed based on the polymerase chain reaction. As little as 1 fg of viral RNA (about 200 target copies) or 10 fg of purified virus (approximately 130 viral particles) could be detected. 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Meristems (0.10 mm to 1.00 mm) were excised from axillary shoots of CyMV and ORSV infected Mokara Char Kuan 'Pink', a monopodial hybrid and CyMV-infected Cattleya Bowbells, a sympodial hybrid. Although the survival rate was very low, two plants were obtained from the larger meristems. However, these plants remained virus-infected. In contrast, thin section culture of both shoot and protocorm-like bodies yielded many virus-free plants regardless of the presence of ribavirin. A highly sensitive diagnostic test for CyMV was also developed based on the polymerase chain reaction. As little as 1 fg of viral RNA (about 200 target copies) or 10 fg of purified virus (approximately 130 viral particles) could be detected. 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