{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/17539"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/17539","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"Investigation of Interleukin 1B synthesis and secretion by dendritic cells: Interplay between toll-like receptor and FCG receptor","abstract":"Interleukin-1I? (IL-1I?) is a prototypic multifunctional cytokine. It not only mediates inflammatory response in innate immunity, but also modulates antigen-specific immunity. Dendritic cells (DCs) are central regulator of immune activation and tolerance. However, the regulation of IL-1I? production in DCs is less clear. In this project, the underlying regulatory mechanisms of IL-1I? synthesis and secretion in monocyte-derived DCs (moDCs) are investigated. It was observed that, LPS stimulation induced moDCs to express IL-1I? mRNA, synthesize pro-IL-1I? and process IL-1I?i? precursors, but with little mature IL-1I? secretion; immobilized IgG (imIgG) stimulation induced little IL-1I? mRNA expression and protein synthesis by moDCs. However, co-stimulation with LPS and imIgG resulted in dramatic increase in IL-1I? secretion from moDCs. The increase was mediated through P2X7 receptor-dependent Ca2+ influx. In addition, Rac1, JNK and MEK were identified as key molecules in LPS/imIgG-induced IL-1I? production and secretion by moDCs, whilst PI3-K and p38 MAPK as negative modulators.","abstract_html":"Interleukin-1I? (IL-1I?) is a prototypic multifunctional cytokine. It not only mediates inflammatory response in innate immunity, but also modulates antigen-specific immunity. Dendritic cells (DCs) are central regulator of immune activation and tolerance. However, the regulation of IL-1I? production in DCs is less clear. In this project, the underlying regulatory mechanisms of IL-1I? synthesis and secretion in monocyte-derived DCs (moDCs) are investigated. It was observed that, LPS stimulation induced moDCs to express IL-1I? mRNA, synthesize pro-IL-1I? and process IL-1I?i? precursors, but with little mature IL-1I? secretion; immobilized IgG (imIgG) stimulation induced little IL-1I? mRNA expression and protein synthesis by moDCs. However, co-stimulation with LPS and imIgG resulted in dramatic increase in IL-1I? secretion from moDCs. The increase was mediated through P2X7 receptor-dependent Ca2+ influx. In addition, Rac1, JNK and MEK were identified as key molecules in LPS/imIgG-induced IL-1I? production and secretion by moDCs, whilst PI3-K and p38 MAPK as negative modulators.","abstract_has_math":false,"creators":["WU XIAOWEI"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007-04-12","date_published":"2007-04-12","updated_at":"2026-07-24T03:31:51Z","subjects":["Interleukin 1B; dendritic cells; Fc gamma receptor; P2X7 receptor;"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["WU XIAOWEI"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2007-04-12"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/17539"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Interleukin 1B; dendritic cells; Fc gamma receptor; P2X7 receptor;"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/bb56a6ee-f97c-4f32-a2a2-4f1e7c10e237/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Interleukin-1I? (IL-1I?) is a prototypic multifunctional cytokine. It not only mediates inflammatory response in innate immunity, but also modulates antigen-specific immunity. Dendritic cells (DCs) are central regulator of immune activation and tolerance. However, the regulation of IL-1I? production in DCs is less clear. In this project, the underlying regulatory mechanisms of IL-1I? synthesis and secretion in monocyte-derived DCs (moDCs) are investigated. It was observed that, LPS stimulation induced moDCs to express IL-1I? mRNA, synthesize pro-IL-1I? and process IL-1I?i? precursors, but with little mature IL-1I? secretion; immobilized IgG (imIgG) stimulation induced little IL-1I? mRNA expression and protein synthesis by moDCs. However, co-stimulation with LPS and imIgG resulted in dramatic increase in IL-1I? secretion from moDCs. The increase was mediated through P2X7 receptor-dependent Ca2+ influx. In addition, Rac1, JNK and MEK were identified as key molecules in LPS/imIgG-induced IL-1I? production and secretion by moDCs, whilst PI3-K and p38 MAPK as negative modulators."]},{"key":"dc:format.checksum.md5","label":"Dc Format Checksum Md5","values":["443b248b8453d8d0f78bbba701ddf442","f20199dc84258e3010f2302e72317406"]},{"key":"dc:title","label":"Title","values":["Investigation of Interleukin 1B synthesis and secretion by dendritic cells: Interplay between toll-like receptor and FCG receptor"]}]}],"canonical_facts":{"dc:creator":["WU XIAOWEI"],"dc:date.issued":["2007-04-12"],"dc:description.abstract":["Interleukin-1I? (IL-1I?) is a prototypic multifunctional cytokine. It not only mediates inflammatory response in innate immunity, but also modulates antigen-specific immunity. Dendritic cells (DCs) are central regulator of immune activation and tolerance. However, the regulation of IL-1I? production in DCs is less clear. In this project, the underlying regulatory mechanisms of IL-1I? synthesis and secretion in monocyte-derived DCs (moDCs) are investigated. It was observed that, LPS stimulation induced moDCs to express IL-1I? mRNA, synthesize pro-IL-1I? and process IL-1I?i? precursors, but with little mature IL-1I? secretion; immobilized IgG (imIgG) stimulation induced little IL-1I? mRNA expression and protein synthesis by moDCs. However, co-stimulation with LPS and imIgG resulted in dramatic increase in IL-1I? secretion from moDCs. The increase was mediated through P2X7 receptor-dependent Ca2+ influx. In addition, Rac1, JNK and MEK were identified as key molecules in LPS/imIgG-induced IL-1I? production and secretion by moDCs, whilst PI3-K and p38 MAPK as negative modulators."],"dc:format.checksum.md5":["443b248b8453d8d0f78bbba701ddf442","f20199dc84258e3010f2302e72317406"],"dc:identifier.uri":["https://scholarbank.nus.edu.sg/bitstreams/bb56a6ee-f97c-4f32-a2a2-4f1e7c10e237/download"],"dc:relation.isreferencedby":["https://scholarbank.nus.edu.sg/handle/10635/17539"],"dc:subject":["Interleukin 1B; dendritic cells; Fc gamma receptor; P2X7 receptor;"],"dc:title":["Investigation of Interleukin 1B synthesis and secretion by dendritic cells: Interplay between toll-like receptor and FCG receptor"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:31:51Z"}