{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/17032"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/17032","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"Osteogenic and chondrogenic differentiation of rat bone marrow stromal cells in 3D culture with novel PCL-TCP scaffolds","abstract":"The aim of this project was to investigate the efficacy of osteogenic and chondrogenic differentiation of rat bone marrow stromal cells (BMSC) in 3D PCL-TCP scaffolds. PCL-TCP scaffolds fabricated by fused deposition modeling (FDM), are honeycombed, highly reproducible bioresorbable 3D structures with a completely interconnected pore network and custom-tailored porosity and pore size. After expansion in vitro, BMSCs were enzymatically lifted and then seeded with fibrin glue as cell suspensions into PCL-TCP scaffolds. The cell-scaffold constructs were cultured for 3 weeks in vitro. Cell viability, morphology, and distribution were examined by phase-contrast microscope, confocal microscope, MTT assay, and SEM. Osteogenic differentiation was substantiated by Von Kossa staining, heightened expression of ALP and osteocalcin; chondrogenic differentiation was confirmed by alcian blue staining and toluidine blue staining. In conclusion, it was demonstrated that PCL-TCP scaffolds with fibrin glue facilitate proliferation, anchorage, motility, and differentiation of both specialized cells and BMSCs.","abstract_html":"The aim of this project was to investigate the efficacy of osteogenic and chondrogenic differentiation of rat bone marrow stromal cells (BMSC) in 3D PCL-TCP scaffolds. PCL-TCP scaffolds fabricated by fused deposition modeling (FDM), are honeycombed, highly reproducible bioresorbable 3D structures with a completely interconnected pore network and custom-tailored porosity and pore size. After expansion in vitro, BMSCs were enzymatically lifted and then seeded with fibrin glue as cell suspensions into PCL-TCP scaffolds. The cell-scaffold constructs were cultured for 3 weeks in vitro. Cell viability, morphology, and distribution were examined by phase-contrast microscope, confocal microscope, MTT assay, and SEM. Osteogenic differentiation was substantiated by Von Kossa staining, heightened expression of ALP and osteocalcin; chondrogenic differentiation was confirmed by alcian blue staining and toluidine blue staining. In conclusion, it was demonstrated that PCL-TCP scaffolds with fibrin glue facilitate proliferation, anchorage, motility, and differentiation of both specialized cells and BMSCs.","abstract_has_math":false,"creators":["SHI ZHENG"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2005,"date_issued":"2005-04-29","date_published":"2005-04-29","updated_at":"2026-07-24T03:32:18Z","subjects":["Chondrogenic; tissue engineering; osteogenic; PCL-TCP; rat bone marrow stromal cells"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["SHI ZHENG"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2005-04-29"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/17032"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chondrogenic; tissue engineering; osteogenic; PCL-TCP; rat bone marrow stromal cells"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/ea643dd5-7b27-42bc-b313-bc75af8d9d98/download","https://scholarbank.nus.edu.sg/bitstreams/761216c6-83ae-4d7c-937b-e35d9a631bdf/download","https://scholarbank.nus.edu.sg/bitstreams/060dc1c7-b584-4ec9-a718-7ee81340c10b/download","https://scholarbank.nus.edu.sg/bitstreams/748e8ba9-7f6f-4e36-8e9d-8ecd2dbae896/download","https://scholarbank.nus.edu.sg/bitstreams/2c4871b4-8a4a-4191-8c53-7aa01a3257f8/download","https://scholarbank.nus.edu.sg/bitstreams/3cadd882-7d5b-4e13-af7f-762d68c82f6b/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The aim of this project was to investigate the efficacy of osteogenic and chondrogenic differentiation of rat bone marrow stromal cells (BMSC) in 3D PCL-TCP scaffolds. 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