{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/154990"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/154990","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"LDB-1 AND SWI/SNF CHROMATIN REMODELING COMPLEX PARTICIPATE IN THE TRANSCRIPTIONAL ACTIVATION BY C. ELEGANS BLIMP1/PRDM1","abstract":"Caenorhabditis elegans BLMP-1 is the ortholog of the human zinc finger and SET domain-containing transcriptional repressor BLIMP1/PRDM1. BLIMP1/PRDM1 and its orthologs are well studied as transcriptional repressors in many species including humans and mice. Interestingly, we found that in C. elegans, BLMP-1 acts as a direct transcriptional activator of bed-3, a gene required for molting and vulval development, providing the first evidence that BLMP-1 can act as a transcriptional activator in C. elegans. To study the role of BLMP-1 as a transcriptional activator, we performed a RNAi screen and identified three possible interacting partners of BLMP-1. Specifically, we found that F58A3.1/LDB-1, F25H8.2 and ZK1128.5/HAM-3 positively regulate bed-3 in a BLMP-1 dependent manner, and that they do not act upstream of BLMP-1. Moreover, F58A3.1/LDB-1 and ZK1128.5/HAM-3 regulate a bed-3 enhancer fragment containing BLMP-1 binding sites. We confirmed that F58A3.1/LDB-1, ZK1128.5/HAM-3, BLMP-1 proteins, as well as their human orthologs, can physically interact with each other. Finally, we found that F58A3.1/LDB-1 and ZK1128.5/HAM-3 not only participate in the transcriptional activation by BLMP-1, but also in the transcriptional repression by BLMP-1.","abstract_html":"Caenorhabditis elegans BLMP-1 is the ortholog of the human zinc finger and SET domain-containing transcriptional repressor BLIMP1/PRDM1. BLIMP1/PRDM1 and its orthologs are well studied as transcriptional repressors in many species including humans and mice. Interestingly, we found that in C. elegans, BLMP-1 acts as a direct transcriptional activator of bed-3, a gene required for molting and vulval development, providing the first evidence that BLMP-1 can act as a transcriptional activator in C. elegans. To study the role of BLMP-1 as a transcriptional activator, we performed a RNAi screen and identified three possible interacting partners of BLMP-1. Specifically, we found that F58A3.1/LDB-1, F25H8.2 and ZK1128.5/HAM-3 positively regulate bed-3 in a BLMP-1 dependent manner, and that they do not act upstream of BLMP-1. Moreover, F58A3.1/LDB-1 and ZK1128.5/HAM-3 regulate a bed-3 enhancer fragment containing BLMP-1 binding sites. We confirmed that F58A3.1/LDB-1, ZK1128.5/HAM-3, BLMP-1 proteins, as well as their human orthologs, can physically interact with each other. Finally, we found that F58A3.1/LDB-1 and ZK1128.5/HAM-3 not only participate in the transcriptional activation by BLMP-1, but also in the transcriptional repression by BLMP-1.","abstract_has_math":false,"creators":["FONG HEI TUNG"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-01-23","date_published":"2019-01-23","updated_at":"2026-07-24T03:32:18Z","subjects":["LDB-1, SWI/SNF, BLIMP1, PRDM1, C. elegans, transcription"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["FONG HEI TUNG"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2019-01-23"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/154990"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["LDB-1, SWI/SNF, BLIMP1, PRDM1, C. elegans, transcription"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/9553acab-f467-4926-81f0-024e360c7b2d/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Caenorhabditis elegans BLMP-1 is the ortholog of the human zinc finger and SET domain-containing transcriptional repressor BLIMP1/PRDM1. BLIMP1/PRDM1 and its orthologs are well studied as transcriptional repressors in many species including humans and mice. Interestingly, we found that in C. elegans, BLMP-1 acts as a direct transcriptional activator of bed-3, a gene required for molting and vulval development, providing the first evidence that BLMP-1 can act as a transcriptional activator in C. elegans. To study the role of BLMP-1 as a transcriptional activator, we performed a RNAi screen and identified three possible interacting partners of BLMP-1. Specifically, we found that F58A3.1/LDB-1, F25H8.2 and ZK1128.5/HAM-3 positively regulate bed-3 in a BLMP-1 dependent manner, and that they do not act upstream of BLMP-1. Moreover, F58A3.1/LDB-1 and ZK1128.5/HAM-3 regulate a bed-3 enhancer fragment containing BLMP-1 binding sites. We confirmed that F58A3.1/LDB-1, ZK1128.5/HAM-3, BLMP-1 proteins, as well as their human orthologs, can physically interact with each other. Finally, we found that F58A3.1/LDB-1 and ZK1128.5/HAM-3 not only participate in the transcriptional activation by BLMP-1, but also in the transcriptional repression by BLMP-1."]},{"key":"dc:format.checksum.md5","label":"Dc Format Checksum Md5","values":["fe8fb82af1fd4eec4c26b8e452affc02","54d83000541ab3cde7f8c005e849d52a"]},{"key":"dc:title","label":"Title","values":["LDB-1 AND SWI/SNF CHROMATIN REMODELING COMPLEX PARTICIPATE IN THE TRANSCRIPTIONAL ACTIVATION BY C. ELEGANS BLIMP1/PRDM1"]}]}],"canonical_facts":{"dc:creator":["FONG HEI TUNG"],"dc:date.issued":["2019-01-23"],"dc:description.abstract":["Caenorhabditis elegans BLMP-1 is the ortholog of the human zinc finger and SET domain-containing transcriptional repressor BLIMP1/PRDM1. BLIMP1/PRDM1 and its orthologs are well studied as transcriptional repressors in many species including humans and mice. Interestingly, we found that in C. elegans, BLMP-1 acts as a direct transcriptional activator of bed-3, a gene required for molting and vulval development, providing the first evidence that BLMP-1 can act as a transcriptional activator in C. elegans. To study the role of BLMP-1 as a transcriptional activator, we performed a RNAi screen and identified three possible interacting partners of BLMP-1. Specifically, we found that F58A3.1/LDB-1, F25H8.2 and ZK1128.5/HAM-3 positively regulate bed-3 in a BLMP-1 dependent manner, and that they do not act upstream of BLMP-1. Moreover, F58A3.1/LDB-1 and ZK1128.5/HAM-3 regulate a bed-3 enhancer fragment containing BLMP-1 binding sites. We confirmed that F58A3.1/LDB-1, ZK1128.5/HAM-3, BLMP-1 proteins, as well as their human orthologs, can physically interact with each other. Finally, we found that F58A3.1/LDB-1 and ZK1128.5/HAM-3 not only participate in the transcriptional activation by BLMP-1, but also in the transcriptional repression by BLMP-1."],"dc:format.checksum.md5":["fe8fb82af1fd4eec4c26b8e452affc02","54d83000541ab3cde7f8c005e849d52a"],"dc:identifier.uri":["https://scholarbank.nus.edu.sg/bitstreams/9553acab-f467-4926-81f0-024e360c7b2d/download"],"dc:relation.isreferencedby":["https://scholarbank.nus.edu.sg/handle/10635/154990"],"dc:subject":["LDB-1, SWI/SNF, BLIMP1, PRDM1, C. elegans, transcription"],"dc:title":["LDB-1 AND SWI/SNF CHROMATIN REMODELING COMPLEX PARTICIPATE IN THE TRANSCRIPTIONAL ACTIVATION BY C. ELEGANS BLIMP1/PRDM1"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:32:18Z"}