{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/153745"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/153745","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"ENHANCING CARDIAC TRANSDIFFERENTIATION OF FIBROBLASTS USING SINGLE-CELL TRANSCRIPTOMICS ANALYSIS AND HIGH-THROUGHPUT CHEMICAL LIBRARY SCREENING APPOACHES","abstract":"Cardiac transdifferentiation could potentially be used to treat acute myocardial infarction, but it is hampered by the lack of effective experimental protocols. By performing high-throughput chemical library screening, we serendipitously discovered that dimethyl sulfoxide (DMSO) itself improves the efficiency of cardiac transdifferentiation. DMSO treatment increased the percentage of cTnT+ and αMHC-mCherry+ cells by about 4-fold and 6-fold, respectively, and induced higher expression of cardiac genes. Further validations suggest that DMSO could function co-linearly with TGF-β or MEK/ERK signalling pathways, although this remains to be proven empirically. Next, single-cell transcriptomic studies revealed trajectory of cardiac transdifferentiation which includes an unstable intermediate subpopulation that ultimately progresses into three distinct terminal subpopulations - Monocle State 8, 10 and 12. Cells from Monocle State 8 and 12 expressed gene signature resembling fibroblasts and mature cardiomyocytes, respectively. Interestingly, cells from Monocle State 10 possess gene signature of both cardiac and fibroblasts, suggesting incomplete transdifferentiation into cardiomyocytes.","abstract_html":"Cardiac transdifferentiation could potentially be used to treat acute myocardial infarction, but it is hampered by the lack of effective experimental protocols. By performing high-throughput chemical library screening, we serendipitously discovered that dimethyl sulfoxide (DMSO) itself improves the efficiency of cardiac transdifferentiation. DMSO treatment increased the percentage of cTnT+ and αMHC-mCherry+ cells by about 4-fold and 6-fold, respectively, and induced higher expression of cardiac genes. Further validations suggest that DMSO could function co-linearly with TGF-β or MEK/ERK signalling pathways, although this remains to be proven empirically. Next, single-cell transcriptomic studies revealed trajectory of cardiac transdifferentiation which includes an unstable intermediate subpopulation that ultimately progresses into three distinct terminal subpopulations - Monocle State 8, 10 and 12. Cells from Monocle State 8 and 12 expressed gene signature resembling fibroblasts and mature cardiomyocytes, respectively. Interestingly, cells from Monocle State 10 possess gene signature of both cardiac and fibroblasts, suggesting incomplete transdifferentiation into cardiomyocytes.","abstract_has_math":false,"creators":["LIM CHOON KIAT"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-12-26","date_published":"2018-12-26","updated_at":"2026-07-24T03:31:13Z","subjects":["dimethyl sulfoxide, DMSO, cardiac transdifferentiation, single-cell RNA sequencing, fibroblasts, cardiomyocytes"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["LIM CHOON KIAT"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2018-12-26"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/153745"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["dimethyl sulfoxide, DMSO, cardiac transdifferentiation, single-cell RNA sequencing, fibroblasts, cardiomyocytes"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/f8a8389f-beb4-4866-a74a-63c1391774fb/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Cardiac transdifferentiation could potentially be used to treat acute myocardial infarction, but it is hampered by the lack of effective experimental protocols. By performing high-throughput chemical library screening, we serendipitously discovered that dimethyl sulfoxide (DMSO) itself improves the efficiency of cardiac transdifferentiation. DMSO treatment increased the percentage of cTnT+ and αMHC-mCherry+ cells by about 4-fold and 6-fold, respectively, and induced higher expression of cardiac genes. Further validations suggest that DMSO could function co-linearly with TGF-β or MEK/ERK signalling pathways, although this remains to be proven empirically. Next, single-cell transcriptomic studies revealed trajectory of cardiac transdifferentiation which includes an unstable intermediate subpopulation that ultimately progresses into three distinct terminal subpopulations - Monocle State 8, 10 and 12. Cells from Monocle State 8 and 12 expressed gene signature resembling fibroblasts and mature cardiomyocytes, respectively. Interestingly, cells from Monocle State 10 possess gene signature of both cardiac and fibroblasts, suggesting incomplete transdifferentiation into cardiomyocytes."]},{"key":"dc:format.checksum.md5","label":"Dc Format Checksum Md5","values":["4c30b5a69be9c922e209577541b7f578","98968cdd4cac0b32eade57e61bb80971"]},{"key":"dc:title","label":"Title","values":["ENHANCING CARDIAC TRANSDIFFERENTIATION OF FIBROBLASTS USING SINGLE-CELL TRANSCRIPTOMICS ANALYSIS AND HIGH-THROUGHPUT CHEMICAL LIBRARY SCREENING APPOACHES"]}]}],"canonical_facts":{"dc:creator":["LIM CHOON KIAT"],"dc:date.issued":["2018-12-26"],"dc:description.abstract":["Cardiac transdifferentiation could potentially be used to treat acute myocardial infarction, but it is hampered by the lack of effective experimental protocols. By performing high-throughput chemical library screening, we serendipitously discovered that dimethyl sulfoxide (DMSO) itself improves the efficiency of cardiac transdifferentiation. DMSO treatment increased the percentage of cTnT+ and αMHC-mCherry+ cells by about 4-fold and 6-fold, respectively, and induced higher expression of cardiac genes. Further validations suggest that DMSO could function co-linearly with TGF-β or MEK/ERK signalling pathways, although this remains to be proven empirically. Next, single-cell transcriptomic studies revealed trajectory of cardiac transdifferentiation which includes an unstable intermediate subpopulation that ultimately progresses into three distinct terminal subpopulations - Monocle State 8, 10 and 12. Cells from Monocle State 8 and 12 expressed gene signature resembling fibroblasts and mature cardiomyocytes, respectively. Interestingly, cells from Monocle State 10 possess gene signature of both cardiac and fibroblasts, suggesting incomplete transdifferentiation into cardiomyocytes."],"dc:format.checksum.md5":["4c30b5a69be9c922e209577541b7f578","98968cdd4cac0b32eade57e61bb80971"],"dc:identifier.uri":["https://scholarbank.nus.edu.sg/bitstreams/f8a8389f-beb4-4866-a74a-63c1391774fb/download"],"dc:relation.isreferencedby":["https://scholarbank.nus.edu.sg/handle/10635/153745"],"dc:subject":["dimethyl sulfoxide, DMSO, cardiac transdifferentiation, single-cell RNA sequencing, fibroblasts, cardiomyocytes"],"dc:title":["ENHANCING CARDIAC TRANSDIFFERENTIATION OF FIBROBLASTS USING SINGLE-CELL TRANSCRIPTOMICS ANALYSIS AND HIGH-THROUGHPUT CHEMICAL LIBRARY SCREENING APPOACHES"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:31:13Z"}