{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/15338"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/15338","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"Misregulation of Iron Handling Proteins in Excitotoxic Brain Injury","abstract":"The increased iron has been implicated as a major generator of reactive oxygen species (ROS), which can cause neuronal death in neurodegenerative diseases. The present study was also carried out to elucidate the expression of iron handling proteins such as divalent metal transporter-1 (DMT1) isoforms, iron regulatory proteins (IRPs), and ferritin in the kainate lesioned hippocampus.A sustained, upregulation of IRP1, IRP2, DMT1 and -IRE DMT1 protein was detected in astrocytes in the kainate lesioned hippocampus. An increase in ferritin expression, and increased level of ferric iron and ferrous iron were also observed in microglia and oligodendrocytes in the kainate-lesioned hippocampus. Increased expression of the iron transporters, ferroportin-1 and ceruloplasmin was also observed after kainate injection. The misregulation of iron handling proteins would lead to abnormally high iron accumulation in kainate lesioned hippocampus, which generate reactive oxidative stress and further contribute to neuronal cell death.","abstract_html":"The increased iron has been implicated as a major generator of reactive oxygen species (ROS), which can cause neuronal death in neurodegenerative diseases. The present study was also carried out to elucidate the expression of iron handling proteins such as divalent metal transporter-1 (DMT1) isoforms, iron regulatory proteins (IRPs), and ferritin in the kainate lesioned hippocampus.A sustained, upregulation of IRP1, IRP2, DMT1 and -IRE DMT1 protein was detected in astrocytes in the kainate lesioned hippocampus. An increase in ferritin expression, and increased level of ferric iron and ferrous iron were also observed in microglia and oligodendrocytes in the kainate-lesioned hippocampus. Increased expression of the iron transporters, ferroportin-1 and ceruloplasmin was also observed after kainate injection. The misregulation of iron handling proteins would lead to abnormally high iron accumulation in kainate lesioned hippocampus, which generate reactive oxidative stress and further contribute to neuronal cell death.","abstract_has_math":false,"creators":["HUANG EN"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2006,"date_issued":"2006-06-14","date_published":"2006-06-14","updated_at":"2026-07-24T03:31:00Z","subjects":["Iron, iron handling proteins, Kainate, DMT1, ferrotin, electron microscopy"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["HUANG EN"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2006-06-14"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/15338"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Iron, iron handling proteins, Kainate, DMT1, ferrotin, electron microscopy"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/d111b51f-c781-4139-8bf0-83b9faec1eba/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The increased iron has been implicated as a major generator of reactive oxygen species (ROS), which can cause neuronal death in neurodegenerative diseases. The present study was also carried out to elucidate the expression of iron handling proteins such as divalent metal transporter-1 (DMT1) isoforms, iron regulatory proteins (IRPs), and ferritin in the kainate lesioned hippocampus.A sustained, upregulation of IRP1, IRP2, DMT1 and -IRE DMT1 protein was detected in astrocytes in the kainate lesioned hippocampus. An increase in ferritin expression, and increased level of ferric iron and ferrous iron were also observed in microglia and oligodendrocytes in the kainate-lesioned hippocampus. Increased expression of the iron transporters, ferroportin-1 and ceruloplasmin was also observed after kainate injection. The misregulation of iron handling proteins would lead to abnormally high iron accumulation in kainate lesioned hippocampus, which generate reactive oxidative stress and further contribute to neuronal cell death."]},{"key":"dc:format.checksum.md5","label":"Dc Format Checksum Md5","values":["501f9c3f31a273d4e49e8c8d1f87c018","6376555e164ea132333f2b28e8e9a354"]},{"key":"dc:title","label":"Title","values":["Misregulation of Iron Handling Proteins in Excitotoxic Brain Injury"]}]}],"canonical_facts":{"dc:creator":["HUANG EN"],"dc:date.issued":["2006-06-14"],"dc:description.abstract":["The increased iron has been implicated as a major generator of reactive oxygen species (ROS), which can cause neuronal death in neurodegenerative diseases. The present study was also carried out to elucidate the expression of iron handling proteins such as divalent metal transporter-1 (DMT1) isoforms, iron regulatory proteins (IRPs), and ferritin in the kainate lesioned hippocampus.A sustained, upregulation of IRP1, IRP2, DMT1 and -IRE DMT1 protein was detected in astrocytes in the kainate lesioned hippocampus. An increase in ferritin expression, and increased level of ferric iron and ferrous iron were also observed in microglia and oligodendrocytes in the kainate-lesioned hippocampus. Increased expression of the iron transporters, ferroportin-1 and ceruloplasmin was also observed after kainate injection. The misregulation of iron handling proteins would lead to abnormally high iron accumulation in kainate lesioned hippocampus, which generate reactive oxidative stress and further contribute to neuronal cell death."],"dc:format.checksum.md5":["501f9c3f31a273d4e49e8c8d1f87c018","6376555e164ea132333f2b28e8e9a354"],"dc:identifier.uri":["https://scholarbank.nus.edu.sg/bitstreams/d111b51f-c781-4139-8bf0-83b9faec1eba/download"],"dc:relation.isreferencedby":["https://scholarbank.nus.edu.sg/handle/10635/15338"],"dc:subject":["Iron, iron handling proteins, Kainate, DMT1, ferrotin, electron microscopy"],"dc:title":["Misregulation of Iron Handling Proteins in Excitotoxic Brain Injury"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:31:00Z"}