IMPACT OF BIOPHYSICAL CUES ON THE FUNCTIONAL MATURITY OF PLURIPOTENT STEM CELL DERIVED ENDOTHELIAL CELLS INTO ARTERIAL AND VENOUS SUBTYPES
type: CreativeWork
description: Pluripotent stem cell derived endothelial cells (PSC-EC) are an upcoming alternate source for cardio-vascular clinical applications. However, PSC-ECs are functionally heterogeneous and functionally immature compared to primary ECs. Hence, this study aims to functionally mature them to either arterial or venous subtype using biophysical cues. Arterial and venous ECs reside in different microenvironments with varying basement membrane architecture and different shear stress. Thus, this study incorporated a Multi Architectural (MARC) chip to study the effect of substrate topography and a multiplex microfluidic device to study the effect of shear on the functional maturity of PSC-EC into the two EC subtypes. We established that gratings and micro-lenses positively enhanced the arterial phenotype of PSC-ECs. Whereas, a low shear stress ≥ 3.8dyne/cm2 for 40-hours supported an arterial phenotype in PSC-ECs. Both biophysical cues overall had a positive influence towards arterial phenotype.
abstract: Pluripotent stem cell derived endothelial cells (PSC-EC) are an upcoming alternate source for cardio-vascular clinical applications. However, PSC-ECs are functionally heterogeneous and functionally immature compared to primary ECs. Hence, this study aims to functionally mature them to either arterial or venous subtype using biophysical cues. Arterial and venous ECs reside in different microenvironments with varying basement membrane architecture and different shear stress. Thus, this study incorporated a Multi Architectural (MARC) chip to study the effect of substrate topography and a multiplex microfluidic device to study the effect of shear on the functional maturity of PSC-EC into the two EC subtypes. We established that gratings and micro-lenses positively enhanced the arterial phenotype of PSC-ECs. Whereas, a low shear stress ≥ 3.8dyne/cm2 for 40-hours supported an arterial phenotype in PSC-ECs. Both biophysical cues overall had a positive influence towards arterial phenotype.
author: SEEP
keywords: human pluripotent stem cells, endothelial cell, shear stress, substrate topography, functional maturation, environmental cues, arterial specification
date published: 2018-08-14
date modified: 2026-07-24T03:30:34Z
provider: National University of Singapore
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: National University of Singapore