{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/151228"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/151228","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"FABRICATION AND CHARACTERIZATION OF CELL-LADEN 3D PRINTED HYBRID SCAFFOLDS FOR ESOPHAGEAL TISSUE REPAIR","abstract":"Tissue engineering (TE) provides promising results in restoring defected or diseased esophageal tissue functions. Nevertheless, existing fabrications are hardly used to achieve a suitable scaffold for esophageal TE. In this study, a hybrid porous scaffold consisting a layer of electrohydrodynamic jet printed (E-jetted) polycaprolactone (PCL) / Pluronic F127 (F127) fiber mesh, and a layer of electrohydrodynamic sprayed (E-sparyed) printed PCL film, has been applied for esophageal TE application. The E-jetted fiber mesh includes controlled and regular structure with micro-interconnected pores as well as geometrically anisotropic struts along the longitudinal direction. After scaffold fabrication, fibroblast was mixed with collagen before seeding. In addition, as a demonstration, HeLa cells were printed through piezoelectric inkjet printing on porous scaffold precisely with low shear stress to produce homogeneous cell-laden hybrid scaffold. These results demonstrate the potential of the hybrid 3D scaffold in organized tissue reconstruction for esophagus healing.","abstract_html":"Tissue engineering (TE) provides promising results in restoring defected or diseased esophageal tissue functions. Nevertheless, existing fabrications are hardly used to achieve a suitable scaffold for esophageal TE. In this study, a hybrid porous scaffold consisting a layer of electrohydrodynamic jet printed (E-jetted) polycaprolactone (PCL) / Pluronic F127 (F127) fiber mesh, and a layer of electrohydrodynamic sprayed (E-sparyed) printed PCL film, has been applied for esophageal TE application. The E-jetted fiber mesh includes controlled and regular structure with micro-interconnected pores as well as geometrically anisotropic struts along the longitudinal direction. After scaffold fabrication, fibroblast was mixed with collagen before seeding. In addition, as a demonstration, HeLa cells were printed through piezoelectric inkjet printing on porous scaffold precisely with low shear stress to produce homogeneous cell-laden hybrid scaffold. 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In this study, a hybrid porous scaffold consisting a layer of electrohydrodynamic jet printed (E-jetted) polycaprolactone (PCL) / Pluronic F127 (F127) fiber mesh, and a layer of electrohydrodynamic sprayed (E-sparyed) printed PCL film, has been applied for esophageal TE application. The E-jetted fiber mesh includes controlled and regular structure with micro-interconnected pores as well as geometrically anisotropic struts along the longitudinal direction. After scaffold fabrication, fibroblast was mixed with collagen before seeding. In addition, as a demonstration, HeLa cells were printed through piezoelectric inkjet printing on porous scaffold precisely with low shear stress to produce homogeneous cell-laden hybrid scaffold. 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