{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/14799"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/14799","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"The hepatitis B core promoter: Control of viral life cycle events","abstract":"The HBV core promoter transcribes both the pre-core and the pre-genomic mRNAfrom which translation of the HBeAg and HBcAg occurs respectively..This study aimed to investigate the importance of the CP in viral replication. Pairedsamples, pre- and post HBeAg seroconversion, were selected from 14 chronic HBVpatients who had a significant decrease in serum viral load after HBeAgseroconversion implying a drop in viral replication. The CP acitivty from each samplewas quantitated via a luciferase reporter gene. 5a?? RACE was also carried out todistinguish between pre-core and pre-genomic mRNA transcription. Sequenceanalysis showed the presence of previously reported and novel CP mutations whichcaused a change in CP activity. However, the correlation between CP activity andviral replication was not significant. Hence, we conclude that the regulation of HBVreplication is a complex process in which the CP plays a necessary, but not sufficient,role.","abstract_html":"The HBV core promoter transcribes both the pre-core and the pre-genomic mRNAfrom which translation of the HBeAg and HBcAg occurs respectively..This study aimed to investigate the importance of the CP in viral replication. Pairedsamples, pre- and post HBeAg seroconversion, were selected from 14 chronic HBVpatients who had a significant decrease in serum viral load after HBeAgseroconversion implying a drop in viral replication. The CP acitivty from each samplewas quantitated via a luciferase reporter gene. 5a?? RACE was also carried out todistinguish between pre-core and pre-genomic mRNA transcription. Sequenceanalysis showed the presence of previously reported and novel CP mutations whichcaused a change in CP activity. However, the correlation between CP activity andviral replication was not significant. Hence, we conclude that the regulation of HBVreplication is a complex process in which the CP plays a necessary, but not sufficient,role.","abstract_has_math":false,"creators":["ONG SHAIR LING, CARMEN"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2005,"date_issued":"2005-05-25","date_published":"2005-05-25","updated_at":"2026-07-24T03:30:47Z","subjects":["HBV, core promoter, viral replication"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["ONG SHAIR LING, CARMEN"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2005-05-25"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/14799"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["HBV, core promoter, viral replication"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/66bea64a-1bef-4065-9ed4-017cf9fd8c95/download","https://scholarbank.nus.edu.sg/bitstreams/7fab34ba-3653-4bf5-b98e-55b03f35bda3/download","https://scholarbank.nus.edu.sg/bitstreams/69ac05a8-25f3-4bbd-a6f6-4b4867d1b43c/download","https://scholarbank.nus.edu.sg/bitstreams/a87562a6-f344-4ddf-a96f-c191af2b6734/download","https://scholarbank.nus.edu.sg/bitstreams/cbbb0bbe-12af-4104-9546-abedebf44629/download","https://scholarbank.nus.edu.sg/bitstreams/7f77d6c9-fa9c-4823-b560-8da6248f316c/download","https://scholarbank.nus.edu.sg/bitstreams/f7465f52-317d-4919-bd88-32be1d9c9bba/download","https://scholarbank.nus.edu.sg/bitstreams/188f864e-c4bb-4476-93fc-2406b248c37f/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The HBV core promoter transcribes both the pre-core and the pre-genomic mRNAfrom which translation of the HBeAg and HBcAg occurs respectively..This study aimed to investigate the importance of the CP in viral replication. 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