{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/138406"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/138406","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"UNDERSTANDING THE NEUROVIRULENCE OF ENTEROVIRUS 71","abstract":"NITD008, an adenosine nucleoside analog, was shown to fully protect EV71-infected two-week old AG129 mice from morbidity and mortality. NSC-34, a murine motor neuron cell line, was found to be susceptible and permissive to EV71 infection, with S41 strain being the most infectious in NSC-34 cells while MS and C2 strains were impaired at entry, replication and/or egress. EV71 exited NSC-34 cells via autophagic vacuoles. S41-infected AG129 mice exhibited morbidity and mortality while MS- or C2-infected mice were asymptomatic. S41-infected mice had high virus titers and histopathological damage in the CNS and this was not detected in MS-/C2-infected mice. Substitution of the 149th amino acid in VP2 protein from isoleucine to lysine (VP2I149K) in S41 resulted in complete loss of infectivity in NSC-34 cells due to an impairment at entry. Infection of mice with VP2I149K ameliorated morbidity and mortality. Reduced viral titers in the spinal cord and brain were observed.","abstract_html":"NITD008, an adenosine nucleoside analog, was shown to fully protect EV71-infected two-week old AG129 mice from morbidity and mortality. NSC-34, a murine motor neuron cell line, was found to be susceptible and permissive to EV71 infection, with S41 strain being the most infectious in NSC-34 cells while MS and C2 strains were impaired at entry, replication and/or egress. EV71 exited NSC-34 cells via autophagic vacuoles. S41-infected AG129 mice exhibited morbidity and mortality while MS- or C2-infected mice were asymptomatic. S41-infected mice had high virus titers and histopathological damage in the CNS and this was not detected in MS-/C2-infected mice. Substitution of the 149th amino acid in VP2 protein from isoleucine to lysine (VP2I149K) in S41 resulted in complete loss of infectivity in NSC-34 cells due to an impairment at entry. Infection of mice with VP2I149K ameliorated morbidity and mortality. Reduced viral titers in the spinal cord and brain were observed.","abstract_has_math":false,"creators":["YEO HUIMIN"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-08-04","date_published":"2017-08-04","updated_at":"2026-07-24T03:33:09Z","subjects":["EV71, neurovirulence, mouse model, NSC-34, NITD008, AG129"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["YEO HUIMIN"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2017-08-04"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/138406"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["EV71, neurovirulence, mouse model, NSC-34, NITD008, AG129"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/3905c071-2f61-47de-a89f-d3d3c6a3d8c0/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["NITD008, an adenosine nucleoside analog, was shown to fully protect EV71-infected two-week old AG129 mice from morbidity and mortality. NSC-34, a murine motor neuron cell line, was found to be susceptible and permissive to EV71 infection, with S41 strain being the most infectious in NSC-34 cells while MS and C2 strains were impaired at entry, replication and/or egress. EV71 exited NSC-34 cells via autophagic vacuoles. S41-infected AG129 mice exhibited morbidity and mortality while MS- or C2-infected mice were asymptomatic. S41-infected mice had high virus titers and histopathological damage in the CNS and this was not detected in MS-/C2-infected mice. Substitution of the 149th amino acid in VP2 protein from isoleucine to lysine (VP2I149K) in S41 resulted in complete loss of infectivity in NSC-34 cells due to an impairment at entry. Infection of mice with VP2I149K ameliorated morbidity and mortality. Reduced viral titers in the spinal cord and brain were observed."]},{"key":"dc:format.checksum.md5","label":"Dc Format Checksum Md5","values":["95d9f519eec19cd3d020d74c59e29afd","c4322485b23ef88719bb3ecbbcacab68"]},{"key":"dc:title","label":"Title","values":["UNDERSTANDING THE NEUROVIRULENCE OF ENTEROVIRUS 71"]}]}],"canonical_facts":{"dc:creator":["YEO HUIMIN"],"dc:date.issued":["2017-08-04"],"dc:description.abstract":["NITD008, an adenosine nucleoside analog, was shown to fully protect EV71-infected two-week old AG129 mice from morbidity and mortality. NSC-34, a murine motor neuron cell line, was found to be susceptible and permissive to EV71 infection, with S41 strain being the most infectious in NSC-34 cells while MS and C2 strains were impaired at entry, replication and/or egress. EV71 exited NSC-34 cells via autophagic vacuoles. S41-infected AG129 mice exhibited morbidity and mortality while MS- or C2-infected mice were asymptomatic. S41-infected mice had high virus titers and histopathological damage in the CNS and this was not detected in MS-/C2-infected mice. Substitution of the 149th amino acid in VP2 protein from isoleucine to lysine (VP2I149K) in S41 resulted in complete loss of infectivity in NSC-34 cells due to an impairment at entry. Infection of mice with VP2I149K ameliorated morbidity and mortality. Reduced viral titers in the spinal cord and brain were observed."],"dc:format.checksum.md5":["95d9f519eec19cd3d020d74c59e29afd","c4322485b23ef88719bb3ecbbcacab68"],"dc:identifier.uri":["https://scholarbank.nus.edu.sg/bitstreams/3905c071-2f61-47de-a89f-d3d3c6a3d8c0/download"],"dc:relation.isreferencedby":["https://scholarbank.nus.edu.sg/handle/10635/138406"],"dc:subject":["EV71, neurovirulence, mouse model, NSC-34, NITD008, AG129"],"dc:title":["UNDERSTANDING THE NEUROVIRULENCE OF ENTEROVIRUS 71"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:33:09Z"}