{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/13802"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/13802","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"Characterization of metronidazole resistant Helicobacter pylori","abstract":"The high prevalence of metronidazole resistant Helicobacter pylori (MtzR- Hp ) in relation to gastroduodenal disease is of concern. The study shows that MtzR- Hp is significantly higher in non-ulcer dyspepsia patients ( p = 0.005), expresses lower Le b ( p = 0.0365) and babA2 ( p = 0.03). RAPD profiles show the existence of high genetic diversity among strains and suggest a probable involvement of mutations in short segment of gene sequences in MtzR- Hp . rdxA gene showed de novo mutations with no specific mutation sites, rendering it unsuitable as metronidazole resistant marker. Induced MtzR- Hp under sub-optimal metronidazole concentration affected flagellin and adhesin-thiol peroxidase (TP) expression as shown by 2D-PAGE. Furthermore, these MtzR- Hp were non-motile and showed the lack of flagella. It is therefore hypothesized that the different expression of various TP isoforms in the presence of sub-lethal metronidazole concentration may have a role in metronidazole resistance.","abstract_html":"The high prevalence of metronidazole resistant Helicobacter pylori (MtzR- Hp ) in relation to gastroduodenal disease is of concern. The study shows that MtzR- Hp is significantly higher in non-ulcer dyspepsia patients ( p = 0.005), expresses lower Le b ( p = 0.0365) and babA2 ( p = 0.03). RAPD profiles show the existence of high genetic diversity among strains and suggest a probable involvement of mutations in short segment of gene sequences in MtzR- Hp . rdxA gene showed de novo mutations with no specific mutation sites, rendering it unsuitable as metronidazole resistant marker. Induced MtzR- Hp under sub-optimal metronidazole concentration affected flagellin and adhesin-thiol peroxidase (TP) expression as shown by 2D-PAGE. Furthermore, these MtzR- Hp were non-motile and showed the lack of flagella. It is therefore hypothesized that the different expression of various TP isoforms in the presence of sub-lethal metronidazole concentration may have a role in metronidazole resistance.","abstract_has_math":false,"creators":["LUI SOOK YIN"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2004,"date_issued":"2004-03-27","date_published":"2004-03-27","updated_at":"2026-07-24T03:31:38Z","subjects":["<i>Helicobacter pylori</i>, metronidazole resistance, 2D-PAGE, <i>rdxA</i>, flagellin, adhesin-thiol peroxidase"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["LUI SOOK YIN"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2004-03-27"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/13802"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["<i>Helicobacter pylori</i>, metronidazole resistance, 2D-PAGE, <i>rdxA</i>, flagellin, adhesin-thiol peroxidase"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/346779d0-f3d6-41b2-aa18-7ec98f7f0cd3/download","https://scholarbank.nus.edu.sg/bitstreams/63dc5193-fc09-465e-9666-426c3e4c47a6/download","https://scholarbank.nus.edu.sg/bitstreams/89373760-cdb9-4fb7-ae27-fc6d6fc0870f/download","https://scholarbank.nus.edu.sg/bitstreams/60fdcb73-f2b8-42f7-9619-61473c4a2251/download","https://scholarbank.nus.edu.sg/bitstreams/38e17c2e-536f-4266-be9b-eea99a822554/download","https://scholarbank.nus.edu.sg/bitstreams/6b0ada61-7b4c-44f2-b62f-b4498929f9c7/download","https://scholarbank.nus.edu.sg/bitstreams/08c275a5-fb83-4211-91a4-798ae233d1d5/download","https://scholarbank.nus.edu.sg/bitstreams/766a5bda-c353-4baa-af15-66c7d264f53d/download","https://scholarbank.nus.edu.sg/bitstreams/5e058e5b-89c2-4f9f-81d7-c84d7d4b76ba/download","https://scholarbank.nus.edu.sg/bitstreams/88e07800-9a05-434d-99b8-bca1f078f22f/download","https://scholarbank.nus.edu.sg/bitstreams/02ef0706-a553-4eaa-9e83-1e73bd808920/download","https://scholarbank.nus.edu.sg/bitstreams/c8eac04b-8612-4047-a94e-26ab4586ebcc/download","https://scholarbank.nus.edu.sg/bitstreams/7e1f5658-3aee-43c2-b337-c056619ebd9f/download","https://scholarbank.nus.edu.sg/bitstreams/dc983601-bfab-4a5e-8644-bd13422f96d5/download","https://scholarbank.nus.edu.sg/bitstreams/68ae1f2b-99f9-4a0a-be4c-6c1db269eab8/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The high prevalence of metronidazole resistant Helicobacter pylori (MtzR- Hp ) in relation to gastroduodenal disease is of concern. 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