{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/137981"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/137981","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"RESPIRABLE SIRNA TARGETING AT THE NF-KB PATHWAY PROTECTS AGAINST INFLAMMATORY LUNG DISEASES","abstract":"Asthma and chronic obstructive pulmonary disease (COPD) are common inflammatory pulmonary diseases. Cumulative evidences have shown the role of NF-kB signaling pathway in the pathogenesis of asthma and COPD. Ribosomal protein S3 (RPS3) is a subunit of NF-kB complex while receptor-interacting protein 2 (Rip2) is a positive upstream regulator of NF-kB pathway. In my PhD projects, I sought to investigate if RPS3 and Rip2 siRNA could protect against HDM-induced mouse asthma model or CS-induced mouse COPD model. RPS3 and Rip2 siRNA markedly knocked down RPS3 and Rip2 levels in mouse cell lines and mouse lung. RPS3 siRNA abated HDM-induced mucus hypersecretion, cytokine production, and serum IgE elevation. RPS3 and Rip2 siRNA ameliorated neutrophil infiltration and suppressed CS-induced inflammatory and oxidative damage marker levels. Here we reported that RPS3 and Rip2 siRNA ameliorated lung inflammation via the interruption of NF-kB activity, postulating the therapeutic potential of them.","abstract_html":"Asthma and chronic obstructive pulmonary disease (COPD) are common inflammatory pulmonary diseases. Cumulative evidences have shown the role of NF-kB signaling pathway in the pathogenesis of asthma and COPD. Ribosomal protein S3 (RPS3) is a subunit of NF-kB complex while receptor-interacting protein 2 (Rip2) is a positive upstream regulator of NF-kB pathway. In my PhD projects, I sought to investigate if RPS3 and Rip2 siRNA could protect against HDM-induced mouse asthma model or CS-induced mouse COPD model. RPS3 and Rip2 siRNA markedly knocked down RPS3 and Rip2 levels in mouse cell lines and mouse lung. RPS3 siRNA abated HDM-induced mucus hypersecretion, cytokine production, and serum IgE elevation. RPS3 and Rip2 siRNA ameliorated neutrophil infiltration and suppressed CS-induced inflammatory and oxidative damage marker levels. Here we reported that RPS3 and Rip2 siRNA ameliorated lung inflammation via the interruption of NF-kB activity, postulating the therapeutic potential of them.","abstract_has_math":false,"creators":["DONG JINRUI"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-08-04","date_published":"2017-08-04","updated_at":"2026-07-24T03:31:51Z","subjects":["Asthma, COPD, NF-kB, RPS3, Rip2"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["DONG JINRUI"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2017-08-04"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/137981"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Asthma, COPD, NF-kB, RPS3, Rip2"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/4242bae5-9cb7-41bb-9824-d8578a07ad13/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Asthma and chronic obstructive pulmonary disease (COPD) are common inflammatory pulmonary diseases. Cumulative evidences have shown the role of NF-kB signaling pathway in the pathogenesis of asthma and COPD. Ribosomal protein S3 (RPS3) is a subunit of NF-kB complex while receptor-interacting protein 2 (Rip2) is a positive upstream regulator of NF-kB pathway. In my PhD projects, I sought to investigate if RPS3 and Rip2 siRNA could protect against HDM-induced mouse asthma model or CS-induced mouse COPD model. RPS3 and Rip2 siRNA markedly knocked down RPS3 and Rip2 levels in mouse cell lines and mouse lung. RPS3 siRNA abated HDM-induced mucus hypersecretion, cytokine production, and serum IgE elevation. RPS3 and Rip2 siRNA ameliorated neutrophil infiltration and suppressed CS-induced inflammatory and oxidative damage marker levels. 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In my PhD projects, I sought to investigate if RPS3 and Rip2 siRNA could protect against HDM-induced mouse asthma model or CS-induced mouse COPD model. RPS3 and Rip2 siRNA markedly knocked down RPS3 and Rip2 levels in mouse cell lines and mouse lung. RPS3 siRNA abated HDM-induced mucus hypersecretion, cytokine production, and serum IgE elevation. RPS3 and Rip2 siRNA ameliorated neutrophil infiltration and suppressed CS-induced inflammatory and oxidative damage marker levels. Here we reported that RPS3 and Rip2 siRNA ameliorated lung inflammation via the interruption of NF-kB activity, postulating the therapeutic potential of them."],"dc:format.checksum.md5":["8bcdae1434d2c1da0bca9ee699680571","fb39e94d97ede160dd9308a021c5fd44"],"dc:identifier.uri":["https://scholarbank.nus.edu.sg/bitstreams/4242bae5-9cb7-41bb-9824-d8578a07ad13/download"],"dc:relation.isreferencedby":["https://scholarbank.nus.edu.sg/handle/10635/137981"],"dc:subject":["Asthma, COPD, NF-kB, RPS3, Rip2"],"dc:title":["RESPIRABLE SIRNA TARGETING AT THE NF-KB PATHWAY PROTECTS AGAINST INFLAMMATORY LUNG DISEASES"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:31:51Z"}