{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/137561"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/137561","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"A NOVEL MAGNETIC THREE-DIMENSIONAL LEVITATED PRIMARY CELL CULTURE SYSTEM FOR THE DEVELOPMENT OF IN VITRO SALIVARY GLAND-LIKE ORGANOIDS","abstract":"Salivary gland (SG) hypofunction and dry mouth leads to oral complications and impaired quality of life. Recently, researchers have proposed to repair hypo-functional SG via various cell-based approaches in three-dimensional (3D) scaffold-based systems. The majority have nonhuman-based components. Therefore, this project aims to develop SG mini-glands/organoids using a novel 3D xeno-free system (M3DL) that assembles and levitates magnetized primary SG-derived cells (SGDC). To accomplish this, SGDC were isolated, expanded, and characterized by flow cytometry, qPCR and immunofluorescence imaging to assess SG-specific markers. Further, SGDC were assembled in M3DL to generate SG-like 3D organoids for 7 days. The resulting organoids presented increased biocompatibility compared to conventional salispheres in cell viability assays. Moreover, the organoids expressed SG markers from different cellular compartments (acinar/ductal epithelial, myoepithelial and neuronal). Intracellular calcium and α-amylase activity assays showed functional organoids with SG-specific secretory activity. Thus, these outcomes suggest that M3DL is a promising tool to generate SG-like organoids for SG repair.","abstract_html":"Salivary gland (SG) hypofunction and dry mouth leads to oral complications and impaired quality of life. Recently, researchers have proposed to repair hypo-functional SG via various cell-based approaches in three-dimensional (3D) scaffold-based systems. The majority have nonhuman-based components. Therefore, this project aims to develop SG mini-glands/organoids using a novel 3D xeno-free system (M3DL) that assembles and levitates magnetized primary SG-derived cells (SGDC). To accomplish this, SGDC were isolated, expanded, and characterized by flow cytometry, qPCR and immunofluorescence imaging to assess SG-specific markers. Further, SGDC were assembled in M3DL to generate SG-like 3D organoids for 7 days. The resulting organoids presented increased biocompatibility compared to conventional salispheres in cell viability assays. Moreover, the organoids expressed SG markers from different cellular compartments (acinar/ductal epithelial, myoepithelial and neuronal). Intracellular calcium and α-amylase activity assays showed functional organoids with SG-specific secretory activity. Thus, these outcomes suggest that M3DL is a promising tool to generate SG-like organoids for SG repair.","abstract_has_math":false,"creators":["RIASAT HASAN"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-08-24","date_published":"2017-08-24","updated_at":"2026-07-24T03:32:18Z","subjects":["Salivary gland, xerostomia, magnetic levitation, primary cell culture, organoids,"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["RIASAT HASAN"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2017-08-24"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/137561"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Salivary gland, xerostomia, magnetic levitation, primary cell culture, organoids,"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/5e942479-b364-42f7-a70c-07c9dc8726d6/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Salivary gland (SG) hypofunction and dry mouth leads to oral complications and impaired quality of life. Recently, researchers have proposed to repair hypo-functional SG via various cell-based approaches in three-dimensional (3D) scaffold-based systems. The majority have nonhuman-based components. Therefore, this project aims to develop SG mini-glands/organoids using a novel 3D xeno-free system (M3DL) that assembles and levitates magnetized primary SG-derived cells (SGDC). To accomplish this, SGDC were isolated, expanded, and characterized by flow cytometry, qPCR and immunofluorescence imaging to assess SG-specific markers. Further, SGDC were assembled in M3DL to generate SG-like 3D organoids for 7 days. The resulting organoids presented increased biocompatibility compared to conventional salispheres in cell viability assays. Moreover, the organoids expressed SG markers from different cellular compartments (acinar/ductal epithelial, myoepithelial and neuronal). Intracellular calcium and α-amylase activity assays showed functional organoids with SG-specific secretory activity. Thus, these outcomes suggest that M3DL is a promising tool to generate SG-like organoids for SG repair."]},{"key":"dc:format.checksum.md5","label":"Dc Format Checksum Md5","values":["23afdee48f1975cf7bc43662d4631575","511eab1c1180d4a65941aafdc8622c0d"]},{"key":"dc:title","label":"Title","values":["A NOVEL MAGNETIC THREE-DIMENSIONAL LEVITATED PRIMARY CELL CULTURE SYSTEM FOR THE DEVELOPMENT OF IN VITRO SALIVARY GLAND-LIKE ORGANOIDS"]}]}],"canonical_facts":{"dc:creator":["RIASAT HASAN"],"dc:date.issued":["2017-08-24"],"dc:description.abstract":["Salivary gland (SG) hypofunction and dry mouth leads to oral complications and impaired quality of life. Recently, researchers have proposed to repair hypo-functional SG via various cell-based approaches in three-dimensional (3D) scaffold-based systems. The majority have nonhuman-based components. Therefore, this project aims to develop SG mini-glands/organoids using a novel 3D xeno-free system (M3DL) that assembles and levitates magnetized primary SG-derived cells (SGDC). To accomplish this, SGDC were isolated, expanded, and characterized by flow cytometry, qPCR and immunofluorescence imaging to assess SG-specific markers. Further, SGDC were assembled in M3DL to generate SG-like 3D organoids for 7 days. The resulting organoids presented increased biocompatibility compared to conventional salispheres in cell viability assays. Moreover, the organoids expressed SG markers from different cellular compartments (acinar/ductal epithelial, myoepithelial and neuronal). Intracellular calcium and α-amylase activity assays showed functional organoids with SG-specific secretory activity. Thus, these outcomes suggest that M3DL is a promising tool to generate SG-like organoids for SG repair."],"dc:format.checksum.md5":["23afdee48f1975cf7bc43662d4631575","511eab1c1180d4a65941aafdc8622c0d"],"dc:identifier.uri":["https://scholarbank.nus.edu.sg/bitstreams/5e942479-b364-42f7-a70c-07c9dc8726d6/download"],"dc:relation.isreferencedby":["https://scholarbank.nus.edu.sg/handle/10635/137561"],"dc:subject":["Salivary gland, xerostomia, magnetic levitation, primary cell culture, organoids,"],"dc:title":["A NOVEL MAGNETIC THREE-DIMENSIONAL LEVITATED PRIMARY CELL CULTURE SYSTEM FOR THE DEVELOPMENT OF IN VITRO SALIVARY GLAND-LIKE ORGANOIDS"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:32:18Z"}