{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/136745"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/136745","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"BEHAVIOURAL ANALYSIS OF ZEBRAFISH RELAXIN-3A MUTANT","abstract":"The insulin-related neuropeptide relaxin-3 (RLN3) has been implicated in stress responses, feeding and anxiety. Most studies have focused on the peptide encoded by the A- and B-chains, and have been conducted using rodents. This thesis explores the <i>rln3a</i> gene in zebrafish, to test whether these roles are evolutionarily conserved. Using CRISPR/Cas9, a 7 bp deletion was generated in the <i>rln3a</i> locus. Mutants showed mild changes in body weight, anxiety and visuomotor response compared to wildtype siblings. A single nucleotide polymorphism (c.158C>T) was found, resulting in a non-synonymous mutation in the coding sequence of the C-peptide. Fish with the c.158C>T polymorphism had increased activity in visual motor response that was dependent on genetic background. Using mutant fish, there was little evidence for a role in stress responses in the rln3 peptide, similar to rodent studies. Instead, the data suggests a possible function for the C-peptide.","abstract_html":"The insulin-related neuropeptide relaxin-3 (RLN3) has been implicated in stress responses, feeding and anxiety. Most studies have focused on the peptide encoded by the A- and B-chains, and have been conducted using rodents. This thesis explores the &lt;i&gt;rln3a&lt;/i&gt; gene in zebrafish, to test whether these roles are evolutionarily conserved. Using CRISPR/Cas9, a 7 bp deletion was generated in the &lt;i&gt;rln3a&lt;/i&gt; locus. Mutants showed mild changes in body weight, anxiety and visuomotor response compared to wildtype siblings. A single nucleotide polymorphism (c.158C&gt;T) was found, resulting in a non-synonymous mutation in the coding sequence of the C-peptide. Fish with the c.158C&gt;T polymorphism had increased activity in visual motor response that was dependent on genetic background. Using mutant fish, there was little evidence for a role in stress responses in the rln3 peptide, similar to rodent studies. Instead, the data suggests a possible function for the C-peptide.","abstract_has_math":false,"creators":["CHIA SHU MING JOANNE"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-03-30","date_published":"2017-03-30","updated_at":"2026-07-24T03:31:13Z","subjects":["relaxin-3, zebrafish, CRISPR, behaviour, stress response, C-peptide"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["CHIA SHU MING JOANNE"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2017-03-30"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/136745"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["relaxin-3, zebrafish, CRISPR, behaviour, stress response, C-peptide"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/a348d757-5f8b-48ed-a9e6-0c6b76d51feb/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The insulin-related neuropeptide relaxin-3 (RLN3) has been implicated in stress responses, feeding and anxiety. Most studies have focused on the peptide encoded by the A- and B-chains, and have been conducted using rodents. This thesis explores the <i>rln3a</i> gene in zebrafish, to test whether these roles are evolutionarily conserved. Using CRISPR/Cas9, a 7 bp deletion was generated in the <i>rln3a</i> locus. Mutants showed mild changes in body weight, anxiety and visuomotor response compared to wildtype siblings. A single nucleotide polymorphism (c.158C>T) was found, resulting in a non-synonymous mutation in the coding sequence of the C-peptide. Fish with the c.158C>T polymorphism had increased activity in visual motor response that was dependent on genetic background. Using mutant fish, there was little evidence for a role in stress responses in the rln3 peptide, similar to rodent studies. 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A single nucleotide polymorphism (c.158C>T) was found, resulting in a non-synonymous mutation in the coding sequence of the C-peptide. Fish with the c.158C>T polymorphism had increased activity in visual motor response that was dependent on genetic background. Using mutant fish, there was little evidence for a role in stress responses in the rln3 peptide, similar to rodent studies. Instead, the data suggests a possible function for the C-peptide."],"dc:format.checksum.md5":["6b3302ac92d574cda632247e016ed31b","81a5a5641187d8017c86922f9bb76072"],"dc:identifier.uri":["https://scholarbank.nus.edu.sg/bitstreams/a348d757-5f8b-48ed-a9e6-0c6b76d51feb/download"],"dc:relation.isreferencedby":["https://scholarbank.nus.edu.sg/handle/10635/136745"],"dc:subject":["relaxin-3, zebrafish, CRISPR, behaviour, stress response, C-peptide"],"dc:title":["BEHAVIOURAL ANALYSIS OF ZEBRAFISH RELAXIN-3A MUTANT"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T03:31:13Z"}