{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/13389"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/13389","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"Application of HCRSV protein cage for anticancer drug delivery","abstract":"The icosahedral protein cage (PC) of the Hibiscus chlorotic ringspot virus (HCRSV) was explored as a platform for anticancer drug delivery. PC was prepared by removing viral RNA from coat proteins by dialysis against a Ca2+-deficient buffer of pH 8.0, and reassembling the coat proteins by dialysis with a Ca2+-containing buffer of pH 5.0. Using polyacids and dextrans as exemplary cargoes, it was found that only polyacids with MW above 13 kDa were successfully loaded into the PC. A novel method, named a??polyacid associationa??, was developed to load doxorubicin in the PC, which was conjugated with surface folic acid for targeting to cancer cells. The resulting fPC-Dox system had comparable morphology and physical properties as the HCRSV. It showed higher in vitro cytotoxicity against OVCAR-3 and CNE-1 cancer cells than against the representative normal CCL-186 cells, suggesting that the HCRSV PC had potential for targeted anticancer drug delivery.","abstract_html":"The icosahedral protein cage (PC) of the Hibiscus chlorotic ringspot virus (HCRSV) was explored as a platform for anticancer drug delivery. PC was prepared by removing viral RNA from coat proteins by dialysis against a Ca2+-deficient buffer of pH 8.0, and reassembling the coat proteins by dialysis with a Ca2+-containing buffer of pH 5.0. Using polyacids and dextrans as exemplary cargoes, it was found that only polyacids with MW above 13 kDa were successfully loaded into the PC. A novel method, named a??polyacid associationa??, was developed to load doxorubicin in the PC, which was conjugated with surface folic acid for targeting to cancer cells. The resulting fPC-Dox system had comparable morphology and physical properties as the HCRSV. It showed higher in vitro cytotoxicity against OVCAR-3 and CNE-1 cancer cells than against the representative normal CCL-186 cells, suggesting that the HCRSV PC had potential for targeted anticancer drug delivery.","abstract_has_math":false,"creators":["REN YUPENG"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["CHAN LAI WAH"],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007-09-18","date_published":"2007-09-18","updated_at":"2026-08-21T16:47:23Z","subjects":["Plant virus, Hibiscus chlorotic ringspot virus, Protein cage, Anticancer drug, Doxorubicin, Polyacid association"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarbank.nus.edu.sg/handle/10635/13389","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"source_record":{"url":"https://scholarbank.nus.edu.sg/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai%3Ascholarbank.nus.edu.sg%3A10635%2F13389","prefix":"dim"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.other","label":"Dc Contributor Other","values":["PHARMACY"]},{"key":"dc:contributor.supervisor","label":"Supervisor","values":["CHAN LAI WAH"]},{"key":"dc:creator","label":"Author","values":["REN YUPENG"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2010-04-08T10:32:35Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2010-04-08T10:32:35Z"]},{"key":"dc:date.issued","label":"Date","values":["2007-09-18"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://scholarbank.nus.edu.sg/handle/10635/13389"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Plant virus, Hibiscus chlorotic ringspot virus, Protein cage, Anticancer drug, Doxorubicin, Polyacid association"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://scholarbank.nus.edu.sg/bitstreams/f19a2bc1-9b40-49dc-857f-d9bb2ffeb0f2/download","https://scholarbank.nus.edu.sg/handle/10635/13389"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The icosahedral protein cage (PC) of the Hibiscus chlorotic ringspot virus (HCRSV) was explored as a platform for anticancer drug delivery. 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