{"id":{"repo_id":"nus","oai_identifier":"oai:scholarbank.nus.edu.sg:10635/121364"},"canonical_url":"https://search.dev.ndltd.org/etd/nus/oai:scholarbank.nus.edu.sg:10635/121364","repository":{"repo_id":"nus","name":"National University of Singapore","base_url":"https://scholarbank.nus.edu.sg/oai/request"},"display":{"title":"IDENTIFICATION AND QUANTIFICATION OF IONIC POLLUTANTS IN ENVIRONMENTAL WATER","abstract":"This dissertation depicts research efforts aimed at developing analytical methods for the determination of both cationic and anionic pollutants in environmental water. In chapter 2, a DNA-based molecular beacon fluorescent sensor was demonstrated for the detection of a cationic pollutant, namely Hg2+, with tunable detection range. This sensor can easily detect Hg2+ in the presence of other metal ions. It is potentially useful for on-site detection in environmental applications involving different concentration ranges. In chapter 3, a label-free DNAzyme-based quartz crystal microbalance biosensor, with oligonucleotide-functionalized gold nanoparticles-enhancement, was demonstrated for detection of another cationic pollutant, i.e. Pb2+. This biosensor showed high selectivity toward Pb2+ ions. The detection strategy would provide an opportunity for ?pollution-free? and on-line detection of metal ions in water. In chapter 4, a highly sensitive two-dimensional ion chromatography (IC) method was demonstrated for detection of anionic disinfection by-products. This method is fully automated, which would allow for high throughput analysis of disinfection by-products by IC with minimal intervention by operator.","abstract_html":"This dissertation depicts research efforts aimed at developing analytical methods for the determination of both cationic and anionic pollutants in environmental water. In chapter 2, a DNA-based molecular beacon fluorescent sensor was demonstrated for the detection of a cationic pollutant, namely Hg2+, with tunable detection range. This sensor can easily detect Hg2+ in the presence of other metal ions. It is potentially useful for on-site detection in environmental applications involving different concentration ranges. In chapter 3, a label-free DNAzyme-based quartz crystal microbalance biosensor, with oligonucleotide-functionalized gold nanoparticles-enhancement, was demonstrated for detection of another cationic pollutant, i.e. Pb2+. This biosensor showed high selectivity toward Pb2+ ions. The detection strategy would provide an opportunity for ?pollution-free? and on-line detection of metal ions in water. In chapter 4, a highly sensitive two-dimensional ion chromatography (IC) method was demonstrated for detection of anionic disinfection by-products. 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It is potentially useful for on-site detection in environmental applications involving different concentration ranges. In chapter 3, a label-free DNAzyme-based quartz crystal microbalance biosensor, with oligonucleotide-functionalized gold nanoparticles-enhancement, was demonstrated for detection of another cationic pollutant, i.e. Pb2+. This biosensor showed high selectivity toward Pb2+ ions. The detection strategy would provide an opportunity for ?pollution-free? and on-line detection of metal ions in water. In chapter 4, a highly sensitive two-dimensional ion chromatography (IC) method was demonstrated for detection of anionic disinfection by-products. 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