{"id":{"repo_id":"nott-trent","oai_identifier":"oai:irep.ntu.ac.uk:32"},"canonical_url":"https://search.dev.ndltd.org/etd/nott-trent/oai:irep.ntu.ac.uk:32","repository":{"repo_id":"nott-trent","name":"Nottingham Trent University","base_url":"https://irep.ntu.ac.uk/cgi/oai2"},"display":{"title":"Proteomics as a tool for the characterisation of nosocomial pathogens","abstract":"The aim of the present investigation was to develop and assess the potential of various proteomic approaches for the characterisation of two major nosocomial pathogens, S. aureus and C. difficile and to further investigate the intraspecies diversity of C. difficile using a genotypic approach. The surface-associated proteins of S. aureus and intracellular stable ribosomal proteins of C. difficile were analysed by MALDI-TOF-MS and the resulting spectra interrogated using two databases viz. MMU (Waters®) and SARAMIS™ (AnagnosTec) respectively. A total of 134 clinical S. aureus isolates were tested using the MMU database and the MicrobeLynx™ software. All were successfully identified with minor contamination errors that corroborated with 16S rRNA sequencing. By contrast, C. difficile isolates were only partially identified (to the genus level) using the MMU database and protocol. Changes in the matrix solution and use of the new database (SARAMIS™) resulted in the correct identification of all C. difficile isolates and, detailed ultra-structural studies indicated that intracellular proteins were the new diagnostic biomarkers.","abstract_html":"The aim of the present investigation was to develop and assess the potential of various proteomic approaches for the characterisation of two major nosocomial pathogens, S. aureus and C. difficile and to further investigate the intraspecies diversity of C. difficile using a genotypic approach. The surface-associated proteins of S. aureus and intracellular stable ribosomal proteins of C. difficile were analysed by MALDI-TOF-MS and the resulting spectra interrogated using two databases viz. MMU (Waters®) and SARAMIS™ (AnagnosTec) respectively. A total of 134 clinical S. aureus isolates were tested using the MMU database and the MicrobeLynx™ software. All were successfully identified with minor contamination errors that corroborated with 16S rRNA sequencing. By contrast, C. difficile isolates were only partially identified (to the genus level) using the MMU database and protocol. Changes in the matrix solution and use of the new database (SARAMIS™) resulted in the correct identification of all C. difficile isolates and, detailed ultra-structural studies indicated that intracellular proteins were the new diagnostic biomarkers.","abstract_has_math":false,"creators":["Rajakaruna, LK"],"institution":"Nottingham Trent University","degree_name":"phd","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010","date_published":"2010","updated_at":"2026-07-24T06:30:42Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Rajakaruna, LK"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2010"]},{"key":"dc:date.issued","label":"Date","values":["2010"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Nottingham Trent University"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://irep.ntu.ac.uk/id/eprint/32/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://irep.ntu.ac.uk/id/eprint/32/1/216303_rajakaruna.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The aim of the present investigation was to develop and assess the potential of various proteomic approaches for the characterisation of two major nosocomial pathogens, S. aureus and C. difficile and to further investigate the intraspecies diversity of C. difficile using a genotypic approach. The surface-associated proteins of S. aureus and intracellular stable ribosomal proteins of C. difficile were analysed by MALDI-TOF-MS and the resulting spectra interrogated using two databases viz. MMU (Waters®) and SARAMIS™ (AnagnosTec) respectively. A total of 134 clinical S. aureus isolates were tested using the MMU database and the MicrobeLynx™ software. All were successfully identified with minor contamination errors that corroborated with 16S rRNA sequencing. By contrast, C. difficile isolates were only partially identified (to the genus level) using the MMU database and protocol. Changes in the matrix solution and use of the new database (SARAMIS™) resulted in the correct identification of all C. difficile isolates and, detailed ultra-structural studies indicated that intracellular proteins were the new diagnostic biomarkers."]},{"key":"dc:format","label":"Dc Format","values":["text"]},{"key":"dc:title","label":"Title","values":["Proteomics as a tool for the characterisation of nosocomial pathogens"]}]}],"canonical_facts":{"dc:creator":["Rajakaruna, LK"],"dc:date":["2010"],"dc:date.issued":["2010"],"dc:description.abstract":["The aim of the present investigation was to develop and assess the potential of various proteomic approaches for the characterisation of two major nosocomial pathogens, S. aureus and C. difficile and to further investigate the intraspecies diversity of C. difficile using a genotypic approach. The surface-associated proteins of S. aureus and intracellular stable ribosomal proteins of C. difficile were analysed by MALDI-TOF-MS and the resulting spectra interrogated using two databases viz. MMU (Waters®) and SARAMIS™ (AnagnosTec) respectively. A total of 134 clinical S. aureus isolates were tested using the MMU database and the MicrobeLynx™ software. All were successfully identified with minor contamination errors that corroborated with 16S rRNA sequencing. By contrast, C. difficile isolates were only partially identified (to the genus level) using the MMU database and protocol. Changes in the matrix solution and use of the new database (SARAMIS™) resulted in the correct identification of all C. difficile isolates and, detailed ultra-structural studies indicated that intracellular proteins were the new diagnostic biomarkers."],"dc:format":["text"],"dc:identifier.uri":["https://irep.ntu.ac.uk/id/eprint/32/1/216303_rajakaruna.pdf"],"dc:language":["en"],"dc:publisher.institution":["Nottingham Trent University"],"dc:relation.isreferencedby":["https://irep.ntu.ac.uk/id/eprint/32/"],"dc:title":["Proteomics as a tool for the characterisation of nosocomial pathogens"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T06:30:42Z"}