Determining the fitness and competitive advantage of the epidemic ExPEC clone E. coli ST131
type: CreativeWork
description: Extraintestinal pathogenic E. coli (ExPEC) is the major aetiological agent of urinary tract infections (UTIs) in humans. The emergence of the CTX-M producing E. coli ST131 clone represents a major challenge to public health worldwide because of its ability to cause a wide range of difficult-to-treat infections in the healthcare and community settings. The key aim of this study was to characterise the traits that give E. coli ST131 a competitive fitness advantage over other potential ExPEC clones. Comparative phenotypic characterisation of a collection of ExPEC strains showed that there was no difference between ST131 and non-ST131 strains in terms of their growth rates in different culture media, their capacity to associate with, invade and form intracellular bacterial communities within T24 human bladder epithelial cells and their ability to persist within U937 human macrophages. Afterwards, this study tested and compared the metabolic activity of a collection of ST131 and non-ST131 strains using two different testing methodologies: API strips and phenotypic microarray (PM) technology. Our API data showed that ST131 strains had a lower metabolic activity for 5 substrates. Further testing of the metabolic activity of E. coli using phenotypic microarray demonstrated the absence of a specific metabolic profile for ST131 strains suggesting that ST131 is not a metabolically distinct lineage of ExPEC and thus altered metabolism might not contribute to the fitness of this clone. The gene content of a group of E. coli including ST131 and non-ST131 strains was investigated to identify the presence of other loci that are uniquely associated with ST131 H30Rx clade, which involves ST131 isolates belonging to the fimH30 lineage and associated with fluoroquinolones resistance and CTX-M-15 production. Our data identified the presence of 150 loci unique to ST131 H30Rx strains, and the most striking finding at a genomic level was the identification of the secondary flagellar locus …
abstract: Extraintestinal pathogenic E. coli (ExPEC) is the major aetiological agent of urinary tract infections (UTIs) in humans. The emergence of the CTX-M producing E. coli ST131 clone represents a major challenge to public health worldwide because of its ability to cause a wide range of difficult-to-treat infections in the healthcare and community settings. The key aim of this study was to characterise the traits that give E. coli ST131 a competitive fitness advantage over other potential ExPEC clones. Comparative phenotypic characterisation of a collection of ExPEC strains showed that there was no difference between ST131 and non-ST131 strains in terms of their growth rates in different culture media, their capacity to associate with, invade and form intracellular bacterial communities within T24 human bladder epithelial cells and their ability to persist within U937 human macrophages. Afterwards, this study tested and compared the metabolic activity of a collection of ST131 and non-ST131 strains using two different testing methodologies: API strips and phenotypic microarray (PM) technology. Our API data showed that ST131 strains had a lower metabolic activity for 5 substrates. Further testing of the metabolic activity of E. coli using phenotypic microarray demonstrated the absence of a specific metabolic profile for ST131 strains suggesting that ST131 is not a metabolically distinct lineage of ExPEC and thus altered metabolism might not contribute to the fitness of this clone. The gene content of a group of E. coli including ST131 and non-ST131 strains was investigated to identify the presence of other loci that are uniquely associated with ST131 H30Rx clade, which involves ST131 isolates belonging to the fimH30 lineage and associated with fluoroquinolones resistance and CTX-M-15 production. Our data identified the presence of 150 loci unique to ST131 H30Rx strains, and the most striking finding at a genomic level was the identification of the secondary flagellar locus …
author: Alqasim, AS
language: en
date published: 2015-02
date modified: 2026-07-24T06:30:52Z
provider: Nottingham Trent University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Nottingham Trent University