Staphylococcus aureus and Streptococcus pneumoniae are bacteria that commonly colonize healthy individuals without causing disease. Methicillin-resistant S. aureus (MRSA), a more virulent type of S. aureus, is carried by a small percentage of people. These two bacteria have an adversarial relationship both in vitro and in vivo, with S. pneumoniae being able to limit the growth of S. aureus. It has been hypothesized that the relationship between these bacteria may be altered in individuals immunized with the pneumococcal conjugate vaccine, raising concerns that vaccinated individuals may be more likely to carry MRSA. Assessing the carriage rate of these bacteria in both vaccinated and non-vaccinated individuals may provide important information to support or refute this hypothesis. To facilitate this study, we developed a multiplex Real Time Polymerase Chain Reaction (PCR) assay to simultaneously detect S. aureus and S. pneumoniae in the same sample.
","abstract_html":"<p><em>Staphylococcus aureus </em>and <em>Streptococcus pneumoniae</em> are bacteria that commonly colonize healthy individuals without causing disease. Methicillin-resistant <em>S. aureus</em> (MRSA), a more virulent type of <em>S. aureus,</em> is carried by a small percentage of people. These two bacteria have an adversarial relationship both in vitro and in vivo, with <em>S. pneumoniae</em> being able to limit the growth of <em>S. aureus</em>. It has been hypothesized that the relationship between these bacteria may be altered in individuals immunized with the pneumococcal conjugate vaccine, raising concerns that vaccinated individuals may be more likely to carry MRSA. Assessing the carriage rate of these bacteria in both vaccinated and non-vaccinated individuals may provide important information to support or refute this hypothesis. To facilitate this study, we developed a multiplex Real Time Polymerase Chain Reaction (PCR) assay to simultaneously detect <em>S. aureus</em> and <em>S. pneumoniae</em> in the same sample.</p>","abstract_has_math":false,"creators":["Pierpont, Melissa J."],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Clinical Sciences","degree_department":null,"school":null,"contributors":["Dr. Paul Mann"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-12-01T08:00:00Z","date_published":"2016-12-01T08:00:00Z","updated_at":"2026-07-24T03:23:47Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://commons.nmu.edu/theses/116","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Paul Mann"]},{"key":"dc:creator","label":"Author","values":["Pierpont, Melissa J."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2016-12-05T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Clinical Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://commons.nmu.edu/theses/116"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Staphylococcus aureus and Streptococcus pneumoniae are bacteria that commonly colonize healthy individuals without causing disease. Methicillin-resistant S. aureus (MRSA), a more virulent type of S. aureus, is carried by a small percentage of people. These two bacteria have an adversarial relationship both in vitro and in vivo, with S. pneumoniae being able to limit the growth of S. aureus. It has been hypothesized that the relationship between these bacteria may be altered in individuals immunized with the pneumococcal conjugate vaccine, raising concerns that vaccinated individuals may be more likely to carry MRSA. Assessing the carriage rate of these bacteria in both vaccinated and non-vaccinated individuals may provide important information to support or refute this hypothesis. To facilitate this study, we developed a multiplex Real Time Polymerase Chain Reaction (PCR) assay to simultaneously detect S. aureus and S. pneumoniae in the same sample.
"]},{"key":"dc:title","label":"Title","values":["MULTIPLEX REAL TIME PCR and MELT CURVE ASSAY DEVELOPMENT FOR THE SIMULTANEOUS DETECTION AND IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE and STAPHYLOCOCCUS AUREUS"]}]}],"canonical_facts":{"dc:contributor":["Dr. Paul Mann"],"dc:creator":["Pierpont, Melissa J."],"dc:date.available":["2016-12-05T08:00:00Z"],"dc:description.abstract":["Staphylococcus aureus and Streptococcus pneumoniae are bacteria that commonly colonize healthy individuals without causing disease. Methicillin-resistant S. aureus (MRSA), a more virulent type of S. aureus, is carried by a small percentage of people. These two bacteria have an adversarial relationship both in vitro and in vivo, with S. pneumoniae being able to limit the growth of S. aureus. It has been hypothesized that the relationship between these bacteria may be altered in individuals immunized with the pneumococcal conjugate vaccine, raising concerns that vaccinated individuals may be more likely to carry MRSA. Assessing the carriage rate of these bacteria in both vaccinated and non-vaccinated individuals may provide important information to support or refute this hypothesis. To facilitate this study, we developed a multiplex Real Time Polymerase Chain Reaction (PCR) assay to simultaneously detect S. aureus and S. pneumoniae in the same sample.
"],"dc:identifier":["https://commons.nmu.edu/theses/116"],"dc:title":["MULTIPLEX REAL TIME PCR and MELT CURVE ASSAY DEVELOPMENT FOR THE SIMULTANEOUS DETECTION AND IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE and STAPHYLOCOCCUS AUREUS"],"thesis:degree_discipline":["Clinical Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T03:23:47Z"}