GBM tumors are the most aggressive and, unfortunately, the most fatal form of brain cancer. GBM tumors with isocitrate dehydrogenase-1 (IDH1) mutation being expressed, lead to higher survival rates in patients that also have full resection of the tumor and chemotherapy. Without this mutation, it is thought that tumors have a higher expression of the protein Basigin and O6-methylguanine-DNA-methyltrasnferase (MGMT) present, causing it to be more aggressive and less responsive to standard care. The objective of this study was to understand the correlation between IDH1 mutation presence and the expression of Basigin and MGMT. The expression of these proteins was observed in tissues sections from GBM tumors. Proteins were labeled with a fluorescent antibody and imaged with a confocal microscope. The tissue images were then analyzed using Imaris software. It was shown that there was a significant difference between the presence of the IDH1 mutation and Basgin, and also MGMT among all of the tissue blocks.
","abstract_html":"<p>GBM tumors are the most aggressive and, unfortunately, the most fatal form of brain cancer. GBM tumors with isocitrate dehydrogenase-1 (IDH1) mutation being expressed, lead to higher survival rates in patients that also have full resection of the tumor and chemotherapy. Without this mutation, it is thought that tumors have a higher expression of the protein Basigin and O6-methylguanine-DNA-methyltrasnferase (MGMT) present, causing it to be more aggressive and less responsive to standard care. The objective of this study was to understand the correlation between IDH1 mutation presence and the expression of Basigin and MGMT. The expression of these proteins was observed in tissues sections from GBM tumors. Proteins were labeled with a fluorescent antibody and imaged with a confocal microscope. The tissue images were then analyzed using Imaris software. It was shown that there was a significant difference between the presence of the IDH1 mutation and Basgin, and also MGMT among all of the tissue blocks.</p>","abstract_has_math":false,"creators":["Wigand, Amanda M"],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Robert Belton"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-05-01T07:00:00Z","date_published":"2016-05-01T07:00:00Z","updated_at":"2026-07-24T03:23:34Z","subjects":["Glioblastoma Multiforme","GBM","immunohistochemistry","immunofluorescence","Medicine and Health Sciences"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://commons.nmu.edu/theses/92","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Robert Belton"]},{"key":"dc:creator","label":"Author","values":["Wigand, Amanda M"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2016-04-10T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Glioblastoma Multiforme","GBM","immunohistochemistry","immunofluorescence","Medicine and Health Sciences"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://commons.nmu.edu/theses/92"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["GBM tumors are the most aggressive and, unfortunately, the most fatal form of brain cancer. GBM tumors with isocitrate dehydrogenase-1 (IDH1) mutation being expressed, lead to higher survival rates in patients that also have full resection of the tumor and chemotherapy. Without this mutation, it is thought that tumors have a higher expression of the protein Basigin and O6-methylguanine-DNA-methyltrasnferase (MGMT) present, causing it to be more aggressive and less responsive to standard care. The objective of this study was to understand the correlation between IDH1 mutation presence and the expression of Basigin and MGMT. The expression of these proteins was observed in tissues sections from GBM tumors. Proteins were labeled with a fluorescent antibody and imaged with a confocal microscope. The tissue images were then analyzed using Imaris software. It was shown that there was a significant difference between the presence of the IDH1 mutation and Basgin, and also MGMT among all of the tissue blocks.
"]},{"key":"dc:title","label":"Title","values":["ANALYSIS OF TUMOR SPECIFIC PROTEIN EXPRESSION IN GLIOBLASTOMA MULTIFORME (GBMs) TUMORS THROUGH IMMUNOHISTOCHEMISTRY"]}]}],"canonical_facts":{"dc:contributor":["Robert Belton"],"dc:creator":["Wigand, Amanda M"],"dc:date.available":["2016-04-10T07:00:00Z"],"dc:description.abstract":["GBM tumors are the most aggressive and, unfortunately, the most fatal form of brain cancer. GBM tumors with isocitrate dehydrogenase-1 (IDH1) mutation being expressed, lead to higher survival rates in patients that also have full resection of the tumor and chemotherapy. Without this mutation, it is thought that tumors have a higher expression of the protein Basigin and O6-methylguanine-DNA-methyltrasnferase (MGMT) present, causing it to be more aggressive and less responsive to standard care. The objective of this study was to understand the correlation between IDH1 mutation presence and the expression of Basigin and MGMT. The expression of these proteins was observed in tissues sections from GBM tumors. Proteins were labeled with a fluorescent antibody and imaged with a confocal microscope. The tissue images were then analyzed using Imaris software. It was shown that there was a significant difference between the presence of the IDH1 mutation and Basgin, and also MGMT among all of the tissue blocks.
"],"dc:identifier":["https://commons.nmu.edu/theses/92"],"dc:subject":["Glioblastoma Multiforme","GBM","immunohistochemistry","immunofluorescence","Medicine and Health Sciences"],"dc:title":["ANALYSIS OF TUMOR SPECIFIC PROTEIN EXPRESSION IN GLIOBLASTOMA MULTIFORME (GBMs) TUMORS THROUGH IMMUNOHISTOCHEMISTRY"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T03:23:34Z"}