Fabrication of nanofiber scaffolds by electrospinning and it's potential for tissue engineering

Electrospinning is a fabrication process that uses an electric field to control the deposition of polymer fibers on to a target substrate. This electrospinning strategy can be used to fabricate fibrous polymer mats composed of fiber diameters ranging from several microns down to tens of nanometers. This study assesses the potential of electrospinning, as an alternative scaffold fabrication technique for tissue engineering applications. In this study, electrospinning is adapted to produce tissue-engineering scaffolds of two different size ranges composed of non-woven poly-L-lactide (PLLA) nanofibers and as a first study, the potential use of these scaffolds as tissue engineering scaffolds was assessed with the cell proliferation of Mesenchymal stem cells. Electrospun fibers were characterized for fiber diameter, porosity, pore size and its distribution. The electrospun scaffolds achieved a high surface area and porosity. Mesenchymal stem cells (MSC) were seeded on to electrospun PLLA scaffolds having two different fiber diameters. The cell-polymer constructs were cultured under static culture conditions. Cell proliferation study was performed. The results showed that MSC tend to proliferate well on nanofibers than on microfibers.

Name thesis:degree_name

Master of Science in Biomedical Engineering - (M.S.)

Discipline thesis:degree_discipline

Biomedical Engineering

Year

2004

Author dc:creator

• Shanmugasundaram, Shobana

Contributors dc:contributor

• Michael Jaffe
• Treena Livingston Arinzeh
• George Collins

• Nanofiber scaffolds
• Electrospinning
• Tissue engineering
• Biomedical Engineering and Bioengineering

Repository record dc:identifier

https://digitalcommons.njit.edu/theses/567

OAI identifier oai:identifier

oai:digitalcommons.njit.edu:theses-1566