The photophysics properties of lanthanide luminescent probes

A detailed study of the luminescence quantum yield, lifetimes and the number of water molecules coordinated to lanthanide luminescent probes based on biphenylyl-7-amino-4- methyl-2(1H)-quinolinon (Bi-cs124) derivatives have been carried out using a time- resolved fluorescence assay (TRFA). Bi-cs124 was conbined with different chelating agents, specifically diethylene triamine pentaacetic acid (DTPA), ethylene diamine tetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), and 3,6,9,12-Tetrakis carboxymethyl)-3,6,9,12-tetraazatetradecane-1,14-dioic acid (TTHA). The results indicate that Bi-cs 124-TTHA has a longest lifetime and smallest number of coordinated water molecules because TTHA has larger number of chelating groups. The dependence of the luminescence lifetimes on the probes concentrations was investigated. The luminescence lifetime is shorter at higher probes concentrations due to self-quenching. Images of E-coli cells labeled with one of the lanthanide luminescent probes have been obtained by TRFA excited by excimer laser radiation (351 nm) using the total internal reflection fluorescence microscopy (TIRFM). The results indicate feasibility of using lanthanide luminescent probes for time-gated luminescence microscopy.

Name thesis:degree_name

Master of Science in Chemical Engineering - (M.S.)

Discipline thesis:degree_discipline

Chemical, Biological and Pharmaceutical Engineering

Year

2014

Author dc:creator

• Li, Ke

Contributors dc:contributor

• Lev N. Krasnoperov
• Arkady Mustaev
• Alexei Khalizov

• Luminescence quantum yield
• Lanthanide luminescent probes
• Luminescence lifetime
• Chemical Engineering

Repository record dc:identifier

https://digitalcommons.njit.edu/theses/208

OAI identifier oai:identifier

oai:digitalcommons.njit.edu:theses-1207