{"id":{"repo_id":"ncsu","oai_identifier":"oai:repository.lib.ncsu.edu:1840.16/339"},"canonical_url":"https://search.dev.ndltd.org/etd/ncsu/oai:repository.lib.ncsu.edu:1840.16/339","repository":{"repo_id":"ncsu","name":"North Carolina State University","base_url":"https://repository.lib.ncsu.edu/server/oai/request"},"display":{"title":"Keratinocyte and Hepatocyte Growth Proliferation and Adhesion to Helium and Helium/Oxygen Atmospheric Pressure Plasma Treated Polyethylene Terephthalate","abstract":"To improve the surface properties of biomaterials, the effects of changes in surface chemistry and morphology of polyethylene terephthalate (PET) films treated with atmospheric pressure plasma were investigated as a function of cellular growth, proliferation, and adhesion. PET films were subjected to helium and helium/oxygen gas plasmas. The contact angle of the treated films decreased due to plasma etching and possible scission indicating that the surfaces become more hydrophilic. Atomic force microscopy results had a large standard error, however the surface visually showed changes in surface micro and nanoscale roughness corresponding to treatment duration. Keratinocytes were plated on the day of plasma treatment and two and five days after plasma treatment and tested half a day, one, two, three, and six days after plating. The same methodology of plating and testing was also applied to hepatocytes. Cell growth, proliferation, and adhesion were characterized via a fluorescent probe based assay and were correlated with surface chemical and nanostructural features. Both the helium and helium/oxygen plasma-treated PET had little or no effect on cell behavior for both keratinocytes and hepatocytes. The nanoscale surface changes due to the plasma surface treatment are believed to be masked by the protein adherence in the media on the surface of the PET.","abstract_html":"To improve the surface properties of biomaterials, the effects of changes in surface chemistry and morphology of polyethylene terephthalate (PET) films treated with atmospheric pressure plasma were investigated as a function of cellular growth, proliferation, and adhesion. PET films were subjected to helium and helium/oxygen gas plasmas. The contact angle of the treated films decreased due to plasma etching and possible scission indicating that the surfaces become more hydrophilic. Atomic force microscopy results had a large standard error, however the surface visually showed changes in surface micro and nanoscale roughness corresponding to treatment duration. Keratinocytes were plated on the day of plasma treatment and two and five days after plasma treatment and tested half a day, one, two, three, and six days after plating. The same methodology of plating and testing was also applied to hepatocytes. Cell growth, proliferation, and adhesion were characterized via a fluorescent probe based assay and were correlated with surface chemical and nanostructural features. Both the helium and helium/oxygen plasma-treated PET had little or no effect on cell behavior for both keratinocytes and hepatocytes. The nanoscale surface changes due to the plasma surface treatment are believed to be masked by the protein adherence in the media on the surface of the PET.","abstract_has_math":false,"creators":["Christie, Megan Allison"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Mohamed Bourham, Committee Co-Chair","Wendy Krause, Committee Co-Chair","Hechmi Hamouda, Committee Member","Jeffrey Macdonald, Committee Member"],"committee_chairs":[],"committee_members":[],"year":2006,"date_issued":"2006-03-06","date_published":"2006-03-06","updated_at":"2026-08-21T22:21:56Z","subjects":["cell adhesion","cell growth","atmospheric pressure plasma","PET"],"languages":[],"rights":["I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-11022005-223628"],"render_values":[{"text":"etd-11022005-223628","href":null,"code":true}]}]},"links":{"outbound_url":"http://www.lib.ncsu.edu/resolver/1840.16/339","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"source_record":{"url":"https://repository.lib.ncsu.edu/server/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai%3Arepository.lib.ncsu.edu%3A1840.16%2F339","prefix":"dim"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Mohamed Bourham, Committee Co-Chair","Wendy Krause, Committee Co-Chair","Hechmi Hamouda, Committee Member","Jeffrey Macdonald, Committee Member"]},{"key":"dc:creator","label":"Author","values":["Christie, Megan Allison"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2010-04-02T17:54:47Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2010-04-02T17:54:47Z"]},{"key":"dc:date.issued","label":"Date","values":["2006-03-06"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["cell adhesion","cell growth","atmospheric pressure plasma","PET"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-11022005-223628"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://www.lib.ncsu.edu/resolver/1840.16/339"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["North Carolina State University Theses Biomedical Engineering.;North Carolina State University Theses Textile Engineering Chemistry and Science."]},{"key":"dc:description.abstract","label":"Abstract","values":["To improve the surface properties of biomaterials, the effects of changes in surface chemistry and morphology of polyethylene terephthalate (PET) films treated with atmospheric pressure plasma were investigated as a function of cellular growth, proliferation, and adhesion. PET films were subjected to helium and helium/oxygen gas plasmas. The contact angle of the treated films decreased due to plasma etching and possible scission indicating that the surfaces become more hydrophilic. Atomic force microscopy results had a large standard error, however the surface visually showed changes in surface micro and nanoscale roughness corresponding to treatment duration. Keratinocytes were plated on the day of plasma treatment and two and five days after plasma treatment and tested half a day, one, two, three, and six days after plating. The same methodology of plating and testing was also applied to hepatocytes. Cell growth, proliferation, and adhesion were characterized via a fluorescent probe based assay and were correlated with surface chemical and nanostructural features. Both the helium and helium/oxygen plasma-treated PET had little or no effect on cell behavior for both keratinocytes and hepatocytes. The nanoscale surface changes due to the plasma surface treatment are believed to be masked by the protein adherence in the media on the surface of the PET."]},{"key":"dc:format","label":"Dc Format","values":["Thesis (M.S.)--North Carolina State University."]},{"key":"dc:title","label":"Title","values":["Keratinocyte and Hepatocyte Growth Proliferation and Adhesion to Helium and Helium/Oxygen Atmospheric Pressure Plasma Treated Polyethylene Terephthalate"]}]}],"canonical_facts":{"dc:contributor.advisor":["Mohamed Bourham, Committee Co-Chair","Wendy Krause, Committee Co-Chair","Hechmi Hamouda, Committee Member","Jeffrey Macdonald, Committee Member"],"dc:creator":["Christie, Megan Allison"],"dc:date.accessioned":["2010-04-02T17:54:47Z"],"dc:date.available":["2010-04-02T17:54:47Z"],"dc:date.issued":["2006-03-06"],"dc:description":["North Carolina State University Theses Biomedical Engineering.;North Carolina State University Theses Textile Engineering Chemistry and Science."],"dc:description.abstract":["To improve the surface properties of biomaterials, the effects of changes in surface chemistry and morphology of polyethylene terephthalate (PET) films treated with atmospheric pressure plasma were investigated as a function of cellular growth, proliferation, and adhesion. PET films were subjected to helium and helium/oxygen gas plasmas. The contact angle of the treated films decreased due to plasma etching and possible scission indicating that the surfaces become more hydrophilic. Atomic force microscopy results had a large standard error, however the surface visually showed changes in surface micro and nanoscale roughness corresponding to treatment duration. Keratinocytes were plated on the day of plasma treatment and two and five days after plasma treatment and tested half a day, one, two, three, and six days after plating. The same methodology of plating and testing was also applied to hepatocytes. Cell growth, proliferation, and adhesion were characterized via a fluorescent probe based assay and were correlated with surface chemical and nanostructural features. Both the helium and helium/oxygen plasma-treated PET had little or no effect on cell behavior for both keratinocytes and hepatocytes. The nanoscale surface changes due to the plasma surface treatment are believed to be masked by the protein adherence in the media on the surface of the PET."],"dc:format":["Thesis (M.S.)--North Carolina State University."],"dc:identifier.other":["etd-11022005-223628"],"dc:identifier.uri":["http://www.lib.ncsu.edu/resolver/1840.16/339"],"dc:rights":["I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report."],"dc:subject":["cell adhesion","cell growth","atmospheric pressure plasma","PET"],"dc:title":["Keratinocyte and Hepatocyte Growth Proliferation and Adhesion to Helium and Helium/Oxygen Atmospheric Pressure Plasma Treated Polyethylene Terephthalate"]},"updated_at":"2026-08-21T22:21:56Z"}