Rapid quantification of trypsin inhibitors in food and feed formulations with electrospray ionization mass spectrometry

"Presence of anti- nutrients deteriorate the nutritional value of animal foods and feed formulations. The important one being trypsin inhibitors. Trypsin inhibitors in food and feed formulations lower the protein supplement and hence the ultimate consequence is the decrease in growth and development of animals. It is therefore, crucial to monitor trypsin inhibitors in animal food and feed formulations. Research work mentioned in this dissertation is based on the development of rapid, more accurate and robust method for determination of trypsin inhibitor in food and feed formulations. The developed method includes extraction of trypsin inhibitor from food and feed formulation and monitoring the decrease in hydrolysis of Hydroxy Methyl Thio Butanoic Acid (HMTBA) derived oligo lysine with the help of electrospray ionization mass spectrometry (ESI-MS). HMTBA capped oligo lysine was synthesized through a chymotrypsin catalyzed reaction in phosphate buffer. The developed method was favorably compared with the existing method recommended by American Association of Cereal and Chemists (AACC). The second part discusses on the test methods and specifications of unused natural ester oils developed for transformers and similar electrical equipment. Because of lack of internationally recognized test methods to study oxidation stability of natural ester oils for use in transformers and similar electrical equipment, this research work has been aimed at developing appropriate oxidation stability test methods for natural esters and synthetic esters. The test methods include various round robin tests participated by different laboratories from seven countries around the world. The natural ester oils in this work refers to the vegetable oils obtained from seed or other suitable biological methods. These specifications have been adopted in new IEC standard test method (IEC 62770)"--Abstract, page iv.

Name thesis:degree_name

Ph. D. in Chemistry

Grantor

Missouri University of Science and Technology

Author dc:creator

• Panta, Radheshyam

• Antioxidant
• Electrospray Ionization
• Mass Spectrometry
• Natural Ester
• Oxidation Stability
• Trypsin Inhibitors
• Analytical Chemistry

Repository record dc:identifier

https://scholarsmine.mst.edu/doctoral_dissertations/2568

OAI identifier oai:identifier

oai:scholarsmine.mst.edu:doctoral_dissertations-3573