The amino acid and monosaccharide content of the cell walls of Thiobacillus thiooxidans
"The objectives of this investigation were: (a) to culture large quantities of Thiobacillus thiooxidans cells, (b) to find an acceptable method of rupturing the cells, (c) to find an acceptable method of isolating the cell walls, and (d) to analyze the cell wall components, especially the amino acid and sugar components. The strain of T. thiooxidans used for this investigation was American Type Culture Collection Number 8085. The surface active agents Tergitol 08 and Tween 80 were investigated for their ability to reduce the lag period found in the growth of T. thiooxidans. Although a concentration of 12.5 ppm Tween 80 did decrease the lag period, the use of these surfactants was not effective in increasing the amount of cells grown over a specific time period because they reduced the cell yield. Two cell rupture techniques, sonication and pressure cell rupture, coupled with either washing with deionized water or washing treatment with IR-120 and AG-1X2 ion exchange resins, were tested"--Abstract, page ii-iii.
Missouri University of Science and Technology
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