Capillary electrophoresis of pteridines in urine and plants and quantification of pharmaceutical and personal care products in Missouri waste water

<p>"Capillary electrophoresis (CE) is a powerful technique that separates molecules based on their respective charge and hydrodynamic size. The major advantages of CE are high separation efficiency, small sample volume requirements, simple to no sample preparation and often organic solvents are not required for the separation.</p> <p>The work presented in this dissertation utilized capillary electrophoresis for the analysis two different classes of compounds in different matrices. The first project investigated pteridines in urine and plants. The second project investigated pharmaceutical and personal care products (PPCP's) in Missouri waste water.</p> <p>As cancer continues to be one of the top three causes of human fatalities, early cancer screening research continues to gain momentum. One specific category of compounds known as pteridines is a class of compounds with potential linkage to cancer progression and deserves further investigation. Therefore, we constructed and performed a systematic investigation and optimization of a method for urinary pteridine analysis using capillary electrophoresis with laser induced fluorescence. This method is believed to possess the potential for clinical diagnosis of the presence of cancer at earlier stages than current methodologies. In addition, the method was also extended to the investigation of pteridines in plants for nutritional research. Due to the matrix differences, a systematic study was also performed.</p> <p>There is a growing concern with the amount of PPCP's in the environment. One major area is the levels of PPCP's in the waste water. As a result, the detection and quantification of PPCP's in waste water offers valuable insight to the amount of contaminations being introduced. Several methods for detecting and quantifying PPCP's have been developed using LC-MS/MS. However, an alternative technique is urgently needed to conduct PPCP analysis when LC/MS is not available laboratories. Therefore, we have developed a method using CE-UV for the simultaneous analysis of eight different PPCP compounds in untreated waste water. Capillary electrophoresis (CE) offers a simple, fast, and low cost alternative analytical technique to LC/MS for PPCP determination in waste water"--Abstract, page iv.</p>

Name thesis:degree_name

Ph. D. in Chemistry

Grantor

Missouri University of Science and Technology

Year dc:date.available

2016

Author dc:creator

• Gibbons, Stephen E.

• Chemistry

Repository record dc:identifier

https://scholarsmine.mst.edu/doctoral_dissertations/13

OAI identifier oai:identifier

oai:scholarsmine.mst.edu:doctoral_dissertations-1015