{"id":{"repo_id":"montana","oai_identifier":"oai:scholarworks.umt.edu:etd-1057"},"canonical_url":"https://search.dev.ndltd.org/etd/montana/oai:scholarworks.umt.edu:etd-1057","repository":{"repo_id":"montana","name":"University of Montana","base_url":"https://scholarworks.umt.edu/do/oai/"},"display":{"title":"A small RNA regulates RpoS in the Lyme disease spirochete Borrelia burgdorferi","abstract":"We have identified and characterized the first sRNA, DsrABb, in the Lyme disease spirochete, <em>Borrelia burgdorferi</em>; as well as, identified a non-canonical RNA chaperone Hfq. The alternative sigma factor RpoS (sigma 38 or sigma S) plays a central role in the reciprocal regulation of the virulence-associated major outer surface proteins OspC and OspA. Temperature is one of the key environmental signals controlling RpoS, but the molecular mechanism by which the signal is transduced remains unknown. DsrABb post-transcriptionally regulates the alternative sigma factor RpoS in response to an increase in temperature, associated with the tick to mammal transmission signal. A novel 5' end of the <em>rpoS</em> mRNA was identified and DsrABb has the potential of extensively base-pairing with the upstream region of this <em>rpoS</em> transcript. We demonstrate that <em>B. burgdorferi</em> strains lacking DsrABb do not upregulate RpoS and OspC in response to an increase in temperature, but do regulate RpoS and OspC in response to changes in pH and cell density. The 5' and 3' ends of DsrABb were mapped, demonstrating that at least four species exist with sizes ranging from 213 to 352 nucleotides. We demonstrate and characterize in vitro the interaction between DsrA and upstream region of <em>rpoS</em> mRNA. We hypothesize that DsrABb binds to the upstream region of the <em>rpoS</em> mRNA and stimulates translation by releasing the Shine-Dalgarno sequence and start site from a stable secondary structure. Therefore, we postulate that DsrABb is a molecular thermometer regulating RpoS in <em>Borrelia burgdorferi</em>.","abstract_html":"We have identified and characterized the first sRNA, DsrABb, in the Lyme disease spirochete, &lt;em&gt;Borrelia burgdorferi&lt;/em&gt;; as well as, identified a non-canonical RNA chaperone Hfq. The alternative sigma factor RpoS (sigma 38 or sigma S) plays a central role in the reciprocal regulation of the virulence-associated major outer surface proteins OspC and OspA. Temperature is one of the key environmental signals controlling RpoS, but the molecular mechanism by which the signal is transduced remains unknown. DsrABb post-transcriptionally regulates the alternative sigma factor RpoS in response to an increase in temperature, associated with the tick to mammal transmission signal. A novel 5&#x27; end of the &lt;em&gt;rpoS&lt;/em&gt; mRNA was identified and DsrABb has the potential of extensively base-pairing with the upstream region of this &lt;em&gt;rpoS&lt;/em&gt; transcript. We demonstrate that &lt;em&gt;B. burgdorferi&lt;/em&gt; strains lacking DsrABb do not upregulate RpoS and OspC in response to an increase in temperature, but do regulate RpoS and OspC in response to changes in pH and cell density. The 5&#x27; and 3&#x27; ends of DsrABb were mapped, demonstrating that at least four species exist with sizes ranging from 213 to 352 nucleotides. We demonstrate and characterize in vitro the interaction between DsrA and upstream region of &lt;em&gt;rpoS&lt;/em&gt; mRNA. We hypothesize that DsrABb binds to the upstream region of the &lt;em&gt;rpoS&lt;/em&gt; mRNA and stimulates translation by releasing the Shine-Dalgarno sequence and start site from a stable secondary structure. Therefore, we postulate that DsrABb is a molecular thermometer regulating RpoS in &lt;em&gt;Borrelia burgdorferi&lt;/em&gt;.","abstract_has_math":false,"creators":["Lybecker, Meghan Catherine"],"institution":"University of Montana","degree_name":"Doctor of Philosophy (PhD)","degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007-01-01T08:00:00Z","date_published":"2007-01-01T08:00:00Z","updated_at":"2026-07-24T03:10:20Z","subjects":["Borrelia burgdorferi","ncRNA","RpoS","sRNA"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarworks.umt.edu/etd/38","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Lybecker, Meghan Catherine"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:publisher","label":"Institution","values":["University of Montana"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Borrelia burgdorferi","ncRNA","RpoS","sRNA"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarworks.umt.edu/etd/38"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["We have identified and characterized the first sRNA, DsrABb, in the Lyme disease spirochete, <em>Borrelia burgdorferi</em>; as well as, identified a non-canonical RNA chaperone Hfq. The alternative sigma factor RpoS (sigma 38 or sigma S) plays a central role in the reciprocal regulation of the virulence-associated major outer surface proteins OspC and OspA. Temperature is one of the key environmental signals controlling RpoS, but the molecular mechanism by which the signal is transduced remains unknown. DsrABb post-transcriptionally regulates the alternative sigma factor RpoS in response to an increase in temperature, associated with the tick to mammal transmission signal. A novel 5' end of the <em>rpoS</em> mRNA was identified and DsrABb has the potential of extensively base-pairing with the upstream region of this <em>rpoS</em> transcript. We demonstrate that <em>B. burgdorferi</em> strains lacking DsrABb do not upregulate RpoS and OspC in response to an increase in temperature, but do regulate RpoS and OspC in response to changes in pH and cell density. The 5' and 3' ends of DsrABb were mapped, demonstrating that at least four species exist with sizes ranging from 213 to 352 nucleotides. We demonstrate and characterize in vitro the interaction between DsrA and upstream region of <em>rpoS</em> mRNA. We hypothesize that DsrABb binds to the upstream region of the <em>rpoS</em> mRNA and stimulates translation by releasing the Shine-Dalgarno sequence and start site from a stable secondary structure. Therefore, we postulate that DsrABb is a molecular thermometer regulating RpoS in <em>Borrelia burgdorferi</em>."]},{"key":"dc:title","label":"Title","values":["A small RNA regulates RpoS in the Lyme disease spirochete Borrelia burgdorferi"]}]}],"canonical_facts":{"dc:creator":["Lybecker, Meghan Catherine"],"dc:description.abstract":["We have identified and characterized the first sRNA, DsrABb, in the Lyme disease spirochete, <em>Borrelia burgdorferi</em>; as well as, identified a non-canonical RNA chaperone Hfq. The alternative sigma factor RpoS (sigma 38 or sigma S) plays a central role in the reciprocal regulation of the virulence-associated major outer surface proteins OspC and OspA. Temperature is one of the key environmental signals controlling RpoS, but the molecular mechanism by which the signal is transduced remains unknown. DsrABb post-transcriptionally regulates the alternative sigma factor RpoS in response to an increase in temperature, associated with the tick to mammal transmission signal. A novel 5' end of the <em>rpoS</em> mRNA was identified and DsrABb has the potential of extensively base-pairing with the upstream region of this <em>rpoS</em> transcript. We demonstrate that <em>B. burgdorferi</em> strains lacking DsrABb do not upregulate RpoS and OspC in response to an increase in temperature, but do regulate RpoS and OspC in response to changes in pH and cell density. The 5' and 3' ends of DsrABb were mapped, demonstrating that at least four species exist with sizes ranging from 213 to 352 nucleotides. We demonstrate and characterize in vitro the interaction between DsrA and upstream region of <em>rpoS</em> mRNA. We hypothesize that DsrABb binds to the upstream region of the <em>rpoS</em> mRNA and stimulates translation by releasing the Shine-Dalgarno sequence and start site from a stable secondary structure. Therefore, we postulate that DsrABb is a molecular thermometer regulating RpoS in <em>Borrelia burgdorferi</em>."],"dc:identifier":["https://scholarworks.umt.edu/etd/38"],"dc:publisher":["University of Montana"],"dc:subject":["Borrelia burgdorferi","ncRNA","RpoS","sRNA"],"dc:title":["A small RNA regulates RpoS in the Lyme disease spirochete Borrelia burgdorferi"],"dc:type":["Dissertation"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T03:10:20Z"}