{"id":{"repo_id":"mo-state","oai_identifier":"oai:bearworks.missouristate.edu:theses-1148"},"canonical_url":"https://search.dev.ndltd.org/etd/mo-state/oai:bearworks.missouristate.edu:theses-1148","repository":{"repo_id":"mo-state","name":"Missouri State University","base_url":"https://bearworks.missouristate.edu/do/oai/"},"display":{"title":"Susceptibility Testing of Pathogenic Fungi With a Radiometric System","abstract":"A radiomethric method was used to determine the susceptibility of clinical isolates of dimorphic fungi (Histoplasma capsulatum, Blastomyces dermatitidis, Sporothrix schenckii) to selected antifungal agents. Antifungal agents evaluated were Amphotericin B, 5-fluorocytosine, Nystatin, Ketoconazole, Miconazole, Terconazole, and Itraconazole. The instrument used to measure amount of radioactive carbon dioxide generated by fungal metabolism of ¹⁴C-glucose in presence of antifungal agents was the Bactex 460. Mycelial cultures were grown on SAB-DEX agar at 25°C and converted to yeast phase in a chemically defined, liquid medium at 37°C. Yeast inoculum was adjusted to 5x10⁴ cells. Results indicate that the radiometric method provides a reproducible, rapid, and quantitative measurement of the inhibitory effects of antifungal agents on yeast growth in one-third the time required with conventional susceptibility test methods. This radiometric method has potential for routine laboratory use in evaluating antibiotic susceptibility of fungi","abstract_html":"A radiomethric method was used to determine the susceptibility of clinical isolates of dimorphic fungi (Histoplasma capsulatum, Blastomyces dermatitidis, Sporothrix schenckii) to selected antifungal agents. Antifungal agents evaluated were Amphotericin B, 5-fluorocytosine, Nystatin, Ketoconazole, Miconazole, Terconazole, and Itraconazole. The instrument used to measure amount of radioactive carbon dioxide generated by fungal metabolism of ¹⁴C-glucose in presence of antifungal agents was the Bactex 460. Mycelial cultures were grown on SAB-DEX agar at 25°C and converted to yeast phase in a chemically defined, liquid medium at 37°C. Yeast inoculum was adjusted to 5x10⁴ cells. Results indicate that the radiometric method provides a reproducible, rapid, and quantitative measurement of the inhibitory effects of antifungal agents on yeast growth in one-third the time required with conventional susceptibility test methods. This radiometric method has potential for routine laboratory use in evaluating antibiotic susceptibility of fungi","abstract_has_math":false,"creators":["Allen, Rhonda Gay"],"institution":null,"degree_name":"Master of Science in Biology","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Richard Myers"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1986,"date_issued":"1986-12-01T08:00:00Z","date_published":"1986-12-01T08:00:00Z","updated_at":"2026-07-24T03:15:12Z","subjects":["Biology"],"languages":[],"rights":["© Rhonda Gay Allen"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://bearworks.missouristate.edu/theses/147","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Richard Myers"]},{"key":"dc:creator","label":"Author","values":["Allen, Rhonda Gay"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© Rhonda Gay Allen"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://bearworks.missouristate.edu/theses/147"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A radiomethric method was used to determine the susceptibility of clinical isolates of dimorphic fungi (Histoplasma capsulatum, Blastomyces dermatitidis, Sporothrix schenckii) to selected antifungal agents. Antifungal agents evaluated were Amphotericin B, 5-fluorocytosine, Nystatin, Ketoconazole, Miconazole, Terconazole, and Itraconazole. The instrument used to measure amount of radioactive carbon dioxide generated by fungal metabolism of ¹⁴C-glucose in presence of antifungal agents was the Bactex 460. Mycelial cultures were grown on SAB-DEX agar at 25°C and converted to yeast phase in a chemically defined, liquid medium at 37°C. Yeast inoculum was adjusted to 5x10⁴ cells. Results indicate that the radiometric method provides a reproducible, rapid, and quantitative measurement of the inhibitory effects of antifungal agents on yeast growth in one-third the time required with conventional susceptibility test methods. This radiometric method has potential for routine laboratory use in evaluating antibiotic susceptibility of fungi"]},{"key":"dc:title","label":"Title","values":["Susceptibility Testing of Pathogenic Fungi With a Radiometric System"]}]}],"canonical_facts":{"dc:contributor":["Richard Myers"],"dc:creator":["Allen, Rhonda Gay"],"dc:description.abstract":["A radiomethric method was used to determine the susceptibility of clinical isolates of dimorphic fungi (Histoplasma capsulatum, Blastomyces dermatitidis, Sporothrix schenckii) to selected antifungal agents. Antifungal agents evaluated were Amphotericin B, 5-fluorocytosine, Nystatin, Ketoconazole, Miconazole, Terconazole, and Itraconazole. The instrument used to measure amount of radioactive carbon dioxide generated by fungal metabolism of ¹⁴C-glucose in presence of antifungal agents was the Bactex 460. Mycelial cultures were grown on SAB-DEX agar at 25°C and converted to yeast phase in a chemically defined, liquid medium at 37°C. Yeast inoculum was adjusted to 5x10⁴ cells. Results indicate that the radiometric method provides a reproducible, rapid, and quantitative measurement of the inhibitory effects of antifungal agents on yeast growth in one-third the time required with conventional susceptibility test methods. This radiometric method has potential for routine laboratory use in evaluating antibiotic susceptibility of fungi"],"dc:identifier":["https://bearworks.missouristate.edu/theses/147"],"dc:rights":["© Rhonda Gay Allen"],"dc:subject":["Biology"],"dc:title":["Susceptibility Testing of Pathogenic Fungi With a Radiometric System"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science in Biology"]},"updated_at":"2026-07-24T03:15:12Z"}