{"id":{"repo_id":"mo-state","oai_identifier":"oai:bearworks.missouristate.edu:theses-1146"},"canonical_url":"https://search.dev.ndltd.org/etd/mo-state/oai:bearworks.missouristate.edu:theses-1146","repository":{"repo_id":"mo-state","name":"Missouri State University","base_url":"https://bearworks.missouristate.edu/do/oai/"},"display":{"title":"The Production of Nitric Oxide By Murine Peritoneal and Alveolar Macrophages in Response to Candida Albicans Yeast and Hyphae","abstract":"Candida albicans is a frequent opportunistic pathogen of immunocompromised hosts. The switch from the yeast to the hyphal form enhances virulence. Murine peritoneal (PM⏀) and alveolar macrophages (AM⏀) produce a variety of cytokines as well as nitric oxide (NO), a short lived radical gas, in response to C. albicans. PM⏀ and AM⏀ were harvested and stimulated with C. albicans yeast or hyphae alone or a combination of interferon-gamma (IFN-⋎) plus C. albicans yeast to produce NO. IFN-⋎ plus bacterial lipopolysaccharide (LPS) provided a positive control. Inhibition studies were performed using NG-monomethyl-L-arginine, an NO synthase inhibitor. PM⏀ produced detectable levels of NO 18 hr after stimulation with the positive control whereas AM⏀ did not produce detectable levels until 48 hr. Neither PM⏀ or AM⏀ produced detectable levels of NO in response to C. albicans yeast or hyphae alone. In response to C. albicans yeast or hyphae plus IFN-⋎, PM⏀ produced NO at concentrations less than 1 uM. However, AM⏀ produced NO at concentrations approximating 15 uM in response to C. albicans yeast plus IFN-⋎and at concentrations approximating 45 uM in response to C. albicans hyphae plus IFN-⋎. This study indicates that PM⏀ and AM⏀ respond differently to challenge, possibly through different processing pathways.","abstract_html":"Candida albicans is a frequent opportunistic pathogen of immunocompromised hosts. The switch from the yeast to the hyphal form enhances virulence. Murine peritoneal (PM⏀) and alveolar macrophages (AM⏀) produce a variety of cytokines as well as nitric oxide (NO), a short lived radical gas, in response to C. albicans. PM⏀ and AM⏀ were harvested and stimulated with C. albicans yeast or hyphae alone or a combination of interferon-gamma (IFN-⋎) plus C. albicans yeast to produce NO. IFN-⋎ plus bacterial lipopolysaccharide (LPS) provided a positive control. Inhibition studies were performed using NG-monomethyl-L-arginine, an NO synthase inhibitor. PM⏀ produced detectable levels of NO 18 hr after stimulation with the positive control whereas AM⏀ did not produce detectable levels until 48 hr. Neither PM⏀ or AM⏀ produced detectable levels of NO in response to C. albicans yeast or hyphae alone. In response to C. albicans yeast or hyphae plus IFN-⋎, PM⏀ produced NO at concentrations less than 1 uM. However, AM⏀ produced NO at concentrations approximating 15 uM in response to C. albicans yeast plus IFN-⋎and at concentrations approximating 45 uM in response to C. albicans hyphae plus IFN-⋎. This study indicates that PM⏀ and AM⏀ respond differently to challenge, possibly through different processing pathways.","abstract_has_math":false,"creators":["Daniels, Deirdre E."],"institution":null,"degree_name":"Master of Science in Biology","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Richard Myers"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1999,"date_issued":"1999-05-01T07:00:00Z","date_published":"1999-05-01T07:00:00Z","updated_at":"2026-07-24T03:15:12Z","subjects":["Biology"],"languages":[],"rights":["© Deirdre E Daniels"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://bearworks.missouristate.edu/theses/145","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Richard Myers"]},{"key":"dc:creator","label":"Author","values":["Daniels, Deirdre E."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© Deirdre E Daniels"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://bearworks.missouristate.edu/theses/145"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Candida albicans is a frequent opportunistic pathogen of immunocompromised hosts. The switch from the yeast to the hyphal form enhances virulence. Murine peritoneal (PM⏀) and alveolar macrophages (AM⏀) produce a variety of cytokines as well as nitric oxide (NO), a short lived radical gas, in response to C. albicans. PM⏀ and AM⏀ were harvested and stimulated with C. albicans yeast or hyphae alone or a combination of interferon-gamma (IFN-⋎) plus C. albicans yeast to produce NO. IFN-⋎ plus bacterial lipopolysaccharide (LPS) provided a positive control. Inhibition studies were performed using NG-monomethyl-L-arginine, an NO synthase inhibitor. PM⏀ produced detectable levels of NO 18 hr after stimulation with the positive control whereas AM⏀ did not produce detectable levels until 48 hr. Neither PM⏀ or AM⏀ produced detectable levels of NO in response to C. albicans yeast or hyphae alone. In response to C. albicans yeast or hyphae plus IFN-⋎, PM⏀ produced NO at concentrations less than 1 uM. However, AM⏀ produced NO at concentrations approximating 15 uM in response to C. albicans yeast plus IFN-⋎and at concentrations approximating 45 uM in response to C. albicans hyphae plus IFN-⋎. This study indicates that PM⏀ and AM⏀ respond differently to challenge, possibly through different processing pathways."]},{"key":"dc:title","label":"Title","values":["The Production of Nitric Oxide By Murine Peritoneal and Alveolar Macrophages in Response to Candida Albicans Yeast and Hyphae"]}]}],"canonical_facts":{"dc:contributor":["Richard Myers"],"dc:creator":["Daniels, Deirdre E."],"dc:description.abstract":["Candida albicans is a frequent opportunistic pathogen of immunocompromised hosts. The switch from the yeast to the hyphal form enhances virulence. Murine peritoneal (PM⏀) and alveolar macrophages (AM⏀) produce a variety of cytokines as well as nitric oxide (NO), a short lived radical gas, in response to C. albicans. PM⏀ and AM⏀ were harvested and stimulated with C. albicans yeast or hyphae alone or a combination of interferon-gamma (IFN-⋎) plus C. albicans yeast to produce NO. IFN-⋎ plus bacterial lipopolysaccharide (LPS) provided a positive control. Inhibition studies were performed using NG-monomethyl-L-arginine, an NO synthase inhibitor. PM⏀ produced detectable levels of NO 18 hr after stimulation with the positive control whereas AM⏀ did not produce detectable levels until 48 hr. Neither PM⏀ or AM⏀ produced detectable levels of NO in response to C. albicans yeast or hyphae alone. In response to C. albicans yeast or hyphae plus IFN-⋎, PM⏀ produced NO at concentrations less than 1 uM. However, AM⏀ produced NO at concentrations approximating 15 uM in response to C. albicans yeast plus IFN-⋎and at concentrations approximating 45 uM in response to C. albicans hyphae plus IFN-⋎. This study indicates that PM⏀ and AM⏀ respond differently to challenge, possibly through different processing pathways."],"dc:identifier":["https://bearworks.missouristate.edu/theses/145"],"dc:rights":["© Deirdre E Daniels"],"dc:subject":["Biology"],"dc:title":["The Production of Nitric Oxide By Murine Peritoneal and Alveolar Macrophages in Response to Candida Albicans Yeast and Hyphae"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science in Biology"]},"updated_at":"2026-07-24T03:15:12Z"}