{"id":{"repo_id":"mo-state","oai_identifier":"oai:bearworks.missouristate.edu:theses-1106"},"canonical_url":"https://search.dev.ndltd.org/etd/mo-state/oai:bearworks.missouristate.edu:theses-1106","repository":{"repo_id":"mo-state","name":"Missouri State University","base_url":"https://bearworks.missouristate.edu/do/oai/"},"display":{"title":"Hydrogen Gas Production By a Species of Ectothiorhodospira","abstract":"A hydrogen gas (H₂) producing species of Ectothiorhodospira isolated from Soap Lake, Washington, possessed the nitrogenase enzyme complex. Increasing evolution of H₂ with decreasing ammonium chloride (NH₄C1) concentrations provided evidence that nitrogenase was the catalyst in gas production. Cells were grown in a mineral medium plus 0.2% acetate with sodium sulfide (Na₂S*9H₂0) as electron donor. Syringes inserted into 27 ml stoppered tubes were used to collect the gas. Factors increasing H₂ production included addition of poor nitrogen sources such as glutamic acid, alanine, and serine; addition of reduced carbon compounds such as propionate and succinate; increasing reducing power by increasing sodium sulfide concentration; and increasing the energy charge (ATP) by increasing light intensity. The use of phototrophs in waste treatment with the production of useful by-products such as H₂ is of interest in industrial research.","abstract_html":"A hydrogen gas (H₂) producing species of Ectothiorhodospira isolated from Soap Lake, Washington, possessed the nitrogenase enzyme complex. Increasing evolution of H₂ with decreasing ammonium chloride (NH₄C1) concentrations provided evidence that nitrogenase was the catalyst in gas production. Cells were grown in a mineral medium plus 0.2% acetate with sodium sulfide (Na₂S*9H₂0) as electron donor. Syringes inserted into 27 ml stoppered tubes were used to collect the gas. Factors increasing H₂ production included addition of poor nitrogen sources such as glutamic acid, alanine, and serine; addition of reduced carbon compounds such as propionate and succinate; increasing reducing power by increasing sodium sulfide concentration; and increasing the energy charge (ATP) by increasing light intensity. The use of phototrophs in waste treatment with the production of useful by-products such as H₂ is of interest in industrial research.","abstract_has_math":false,"creators":["Chadwick, Laurie J."],"institution":null,"degree_name":"Master of Science in Biology","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Roar Irgens"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1986,"date_issued":"1986-12-01T08:00:00Z","date_published":"1986-12-01T08:00:00Z","updated_at":"2026-07-24T03:15:12Z","subjects":["Biology"],"languages":[],"rights":["© Laurie J Chadwick"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://bearworks.missouristate.edu/theses/105","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Roar Irgens"]},{"key":"dc:creator","label":"Author","values":["Chadwick, Laurie J."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© Laurie J Chadwick"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://bearworks.missouristate.edu/theses/105"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A hydrogen gas (H₂) producing species of Ectothiorhodospira isolated from Soap Lake, Washington, possessed the nitrogenase enzyme complex. Increasing evolution of H₂ with decreasing ammonium chloride (NH₄C1) concentrations provided evidence that nitrogenase was the catalyst in gas production. Cells were grown in a mineral medium plus 0.2% acetate with sodium sulfide (Na₂S*9H₂0) as electron donor. Syringes inserted into 27 ml stoppered tubes were used to collect the gas. Factors increasing H₂ production included addition of poor nitrogen sources such as glutamic acid, alanine, and serine; addition of reduced carbon compounds such as propionate and succinate; increasing reducing power by increasing sodium sulfide concentration; and increasing the energy charge (ATP) by increasing light intensity. The use of phototrophs in waste treatment with the production of useful by-products such as H₂ is of interest in industrial research."]},{"key":"dc:title","label":"Title","values":["Hydrogen Gas Production By a Species of Ectothiorhodospira"]}]}],"canonical_facts":{"dc:contributor":["Roar Irgens"],"dc:creator":["Chadwick, Laurie J."],"dc:description.abstract":["A hydrogen gas (H₂) producing species of Ectothiorhodospira isolated from Soap Lake, Washington, possessed the nitrogenase enzyme complex. Increasing evolution of H₂ with decreasing ammonium chloride (NH₄C1) concentrations provided evidence that nitrogenase was the catalyst in gas production. Cells were grown in a mineral medium plus 0.2% acetate with sodium sulfide (Na₂S*9H₂0) as electron donor. Syringes inserted into 27 ml stoppered tubes were used to collect the gas. Factors increasing H₂ production included addition of poor nitrogen sources such as glutamic acid, alanine, and serine; addition of reduced carbon compounds such as propionate and succinate; increasing reducing power by increasing sodium sulfide concentration; and increasing the energy charge (ATP) by increasing light intensity. The use of phototrophs in waste treatment with the production of useful by-products such as H₂ is of interest in industrial research."],"dc:identifier":["https://bearworks.missouristate.edu/theses/105"],"dc:rights":["© Laurie J Chadwick"],"dc:subject":["Biology"],"dc:title":["Hydrogen Gas Production By a Species of Ectothiorhodospira"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science in Biology"]},"updated_at":"2026-07-24T03:15:12Z"}