{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/97273"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/97273","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine","abstract":"ATP-powered proteases enforce protein quality-control and regulation in all domains of life. ClpX, a AAA+ ring homohexamer, uses the energy of ATP binding and hydrolysis to power conformational changes that unfold and translocate target proteins into the ClpP peptidase for degradation. X-ray crystal structures show that some ClpX subunits occupy nucleotide-loadable conformations and others occupy unloadable conformations. Furthermore, biochemical evidence suggests that multiple classes of nucleotide-loadable subunits exist. How asymmetry among subunits is coordinated to achieve mechanical function has remained unclear. Using a combination of mutagenesis, disulfide crosslinking, and fluorescence methods to assay the conformations and nucleotide-binding properties of individual subunits, we demonstrate dynamic interconversion of loadable and unloadable subunits. Such interconversion is required to couple ATP hydrolysis by ClpX to mechanical work, plays a role in substrate binding and ClpP interaction, and is not strictly coupled to the ATP hydrolysis cycle. ATP binding to different classes of subunits drives allosteric changes in ring conformation to allow hydrolysis and coupled machine function, and we present a subunit-specific single molecule nucleotide occupancy assay to elucidate details of this process.","abstract_html":"ATP-powered proteases enforce protein quality-control and regulation in all domains of life. ClpX, a AAA+ ring homohexamer, uses the energy of ATP binding and hydrolysis to power conformational changes that unfold and translocate target proteins into the ClpP peptidase for degradation. X-ray crystal structures show that some ClpX subunits occupy nucleotide-loadable conformations and others occupy unloadable conformations. Furthermore, biochemical evidence suggests that multiple classes of nucleotide-loadable subunits exist. How asymmetry among subunits is coordinated to achieve mechanical function has remained unclear. Using a combination of mutagenesis, disulfide crosslinking, and fluorescence methods to assay the conformations and nucleotide-binding properties of individual subunits, we demonstrate dynamic interconversion of loadable and unloadable subunits. Such interconversion is required to couple ATP hydrolysis by ClpX to mechanical work, plays a role in substrate binding and ClpP interaction, and is not strictly coupled to the ATP hydrolysis cycle. ATP binding to different classes of subunits drives allosteric changes in ring conformation to allow hydrolysis and coupled machine function, and we present a subunit-specific single molecule nucleotide occupancy assay to elucidate details of this process.","abstract_has_math":false,"creators":["Stinson, Benjamin M. (Benjamin Michael)"],"institution":"Massachusetts Institute of Technology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Department of Biology.","school":null,"contributors":[],"advisors":["Robert T. Sauer and Tania A. Baker."],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015","date_published":"2015","updated_at":"2026-07-22T22:21:27Z","subjects":["Biology."],"languages":["eng"],"rights":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission."],"rights_urls":["http://dspace.mit.edu/handle/1721.1/7582"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1721.1/97273","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Robert T. Sauer and Tania A. Baker."]},{"key":"dc:contributor.department","label":"Department","values":["Massachusetts Institute of Technology. Department of Biology."]},{"key":"dc:contributor.other","label":"Dc Contributor Other","values":["Massachusetts Institute of Technology. Department of Biology."]},{"key":"dc:creator","label":"Author","values":["Stinson, Benjamin M. (Benjamin Michael)"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-06-10T18:42:07Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-06-10T18:42:07Z"]},{"key":"dc:date.issued","label":"Date","values":["2015"]},{"key":"dc:publisher","label":"Institution","values":["Massachusetts Institute of Technology"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission."]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://dspace.mit.edu/handle/1721.1/7582"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1721.1/97273"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, February 2015.","This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.","Cataloged from student-submitted PDF version of thesis.","Includes bibliographical references."]},{"key":"dc:description.abstract","label":"Abstract","values":["ATP-powered proteases enforce protein quality-control and regulation in all domains of life. ClpX, a AAA+ ring homohexamer, uses the energy of ATP binding and hydrolysis to power conformational changes that unfold and translocate target proteins into the ClpP peptidase for degradation. X-ray crystal structures show that some ClpX subunits occupy nucleotide-loadable conformations and others occupy unloadable conformations. Furthermore, biochemical evidence suggests that multiple classes of nucleotide-loadable subunits exist. How asymmetry among subunits is coordinated to achieve mechanical function has remained unclear. Using a combination of mutagenesis, disulfide crosslinking, and fluorescence methods to assay the conformations and nucleotide-binding properties of individual subunits, we demonstrate dynamic interconversion of loadable and unloadable subunits. Such interconversion is required to couple ATP hydrolysis by ClpX to mechanical work, plays a role in substrate binding and ClpP interaction, and is not strictly coupled to the ATP hydrolysis cycle. ATP binding to different classes of subunits drives allosteric changes in ring conformation to allow hydrolysis and coupled machine function, and we present a subunit-specific single molecule nucleotide occupancy assay to elucidate details of this process."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph. D."]},{"key":"dc:title","label":"Title","values":["Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine"]}]}],"canonical_facts":{"dc:contributor.advisor":["Robert T. Sauer and Tania A. Baker."],"dc:contributor.department":["Massachusetts Institute of Technology. Department of Biology."],"dc:contributor.other":["Massachusetts Institute of Technology. Department of Biology."],"dc:creator":["Stinson, Benjamin M. (Benjamin Michael)"],"dc:date.accessioned":["2015-06-10T18:42:07Z"],"dc:date.available":["2015-06-10T18:42:07Z"],"dc:date.issued":["2015"],"dc:description":["Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, February 2015.","This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.","Cataloged from student-submitted PDF version of thesis.","Includes bibliographical references."],"dc:description.abstract":["ATP-powered proteases enforce protein quality-control and regulation in all domains of life. ClpX, a AAA+ ring homohexamer, uses the energy of ATP binding and hydrolysis to power conformational changes that unfold and translocate target proteins into the ClpP peptidase for degradation. X-ray crystal structures show that some ClpX subunits occupy nucleotide-loadable conformations and others occupy unloadable conformations. Furthermore, biochemical evidence suggests that multiple classes of nucleotide-loadable subunits exist. How asymmetry among subunits is coordinated to achieve mechanical function has remained unclear. Using a combination of mutagenesis, disulfide crosslinking, and fluorescence methods to assay the conformations and nucleotide-binding properties of individual subunits, we demonstrate dynamic interconversion of loadable and unloadable subunits. Such interconversion is required to couple ATP hydrolysis by ClpX to mechanical work, plays a role in substrate binding and ClpP interaction, and is not strictly coupled to the ATP hydrolysis cycle. ATP binding to different classes of subunits drives allosteric changes in ring conformation to allow hydrolysis and coupled machine function, and we present a subunit-specific single molecule nucleotide occupancy assay to elucidate details of this process."],"dc:description.degree":["Ph. D."],"dc:identifier.uri":["http://hdl.handle.net/1721.1/97273"],"dc:language.iso":["eng"],"dc:publisher":["Massachusetts Institute of Technology"],"dc:rights":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission."],"dc:rights.uri":["http://dspace.mit.edu/handle/1721.1/7582"],"dc:subject":["Biology."],"dc:title":["Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine"],"dc:type":["Thesis"]},"updated_at":"2026-07-22T22:21:27Z"}