New immobilized antimicrobial polyethylenimines : synthesis and properties

Surfaces modified with immobilized N-alkyl-polyethylenimines (N-alkyl-PEls) containing various alkyl groups were synthesized and tested against various pathogenic human influenza viruses to establish structure-to-virucidal activity relationships. Various physical-chemical properties of each surface were correlated with their virucidal activities to identify key antiviral surface properties. The accessibility of N-alkyl-PEI quaternary ammonium groups to influenza virus was subsequently identified as the key determinant of antiviral efficacy, as demonstrated by FITC-lysozyme surface titration. Previously used multistep syntheses to create antimicrobial surfaces by immobilizing Nalkyl- PEls were replaced with a novel aerosol-assisted plasma deposition procedure. N,N-hexyl,methyl-polyethylenimines were directly plasma-coated onto a glass surface. The resulting material was thoroughly characterized and demonstrated to be robust, scalable, bactericidal against Escherichia cofi, and virucidal against human influenza virus. Biocompatibility and bactericidal properties of N-alkyl-PEls immobilized on Boston Keratoprosthetic implants were evaluated in vivo. Surface-attached N,N-hexyl,methylpolyethylenimines exhibited inhibitory effects on Staphylococcus aureus biofilm formation, with no toxicity or adverse reactivity detected.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Chemistry.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2014

Author dc:creator

• Liu, Harris K. (Harris Ken-Ming)

Advisor dc:contributor.advisor

• Alexander M. Klibanov.

• Chemistry.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/93039

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/93039