Measurement of blood analytes in turbid biological tissue using near-infrared Raman spectroscopy

Concentrations of blood constituents provide diagnostic information in clinical procedures. Among the medical community, there is great interest in developing an optical method of concentration measurements that eliminates reagents required in the current clinical chemistry techniques and is non-invasive. This thesis describes the methodology to develop a measurement technique of blood component concentrations using Raman spectroscopy. The sources of prediction error were identified. A Monte Carlo simulation model was developed to study the distribution of the Raman signal from turbid biological samples. A high sensitivity system for Raman spectroscopy of blood analytes was designed by optimizing the optical components. The new system had higher collection efficiency than the previous state-of-the-art system. Experiments were performed using the new system in order to collect Raman spectra of human whole blood. The collected Raman spectra were analyzed with multivariate calibration techniques, and compared with hospital measurements. The analysis of the spectra of whole blood samples demonstrate that many chemical components in ex vivo whole blood samples can be measured accurately with near-infrared Raman spectroscopy. A preliminary analysis based on the results of this thesis indicates that it is feasible to measure blood analytes non-invasively.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Mechanical Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2001

Author dc:creator

• Koo, Tae-Woong, 1974-

Advisor dc:contributor.advisor

• Michael S. Feld.

• Mechanical Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/8692

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/8692