Nitric oxide : cellular effects in vitro and in vivo

The overall aim of this project was to investigate various cellular responses and toxic effects of nitric oxide, NO' and in vitro and in vivo. Nitric oxide gives rise to a complex spectrum of reactive species in oxygenated solution. The complexity of nitric oxide's chemistry is recapitulated in its effects on cells. Exposure to nitric oxide can result in changes on many different levels in cells ranging from protein and DNA damage, to damage to organelles and changes in gene expression, and even in cell death. Many models to study nitric oxide have been developed and will be used to study various responses to nitric oxide and related species. Nitric oxide and peroxynitrite-induced cellular damage has been and continues to be studied extensively using in vitro systems. Two systems have been used in this project, a delivery system for NO' as well as a cell line, which produces NO'. A Silasticʾ membrane delivery system can be used to treat bacteria or cells to mimic in vivo exposure to nitric oxide. Mutations induced by nitric oxide in a set of Salmonella tester strains can be studied utilizing this delivery system. Activated RAW264.7 macrophage cells have been used as an in vitro model of nitric oxide production and cytotoxicity SJL/J mice bearing the transplantable lymphoma RcsX have been established as an in vivo model of nitric oxide production and toxicity.

Department dc:contributor.department

Massachusetts Institute of Technology. Division of Bioengineering and Environmental Health

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2001

Author dc:creator

• Wright, Teresa Leah, 1970-

Advisor dc:contributor.advisor

• Steven R. Tannenbaum.

• Division of Bioengineering and Environmental Health.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/8182

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/8182