Development and optimization of high-throughput zebrafish screening platform

The high-throughput zebrafish screening platform is a revolutionary tool that enables subcellular precision in vivo whole animal screening of Danio Rerio. It can perform laser surgery and/or imaging in less than twenty seconds per fish. This is a two orders of magnitude improvement from the existing manual method, which takes 10 to 15 minutes per fish. The platform also has the ability to rotate the fish in any angle for imaging or laser surgery. There has never been a platform that could align and position a zebrafish consistently and efficiently in such manner before. This crucial functionality was realized through a design in which zebrafish was screened in a capillary tube.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2010

Author dc:creator

• Koo, Bryan Kyo

Advisor dc:contributor.advisor

• Mehmet Fatih Yanik.

• Electrical Engineering and Computer Science.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/62394

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/62394