Cyclin-Dependent Kinase regulation and function during meiosis

Meiosis is the process by which haploid gametes are produced from a diploid progenitor cell. Accurate completion of the meiotic divisions requires a variety of modifications to the mitotic chromosome segregation machinery, which allow the reductional meiotic chromosome segregation program to occur. Oscillations in the activity of Cyclin- Dependent Kinases (CDKs) drive virtually every event in the mitotic cell cycle, including events such as cell cycle entry, DNA replication, and chromosome segregation. While much is known about the activity of CDKs, the regulation of CDK activity, and the mechanisms by which CDK activity promotes cell cycle events during vegetative growth in Saccharomyces cerevisiae, relatively little is known about the roles of CDKs during the meiotic divisions. This work examines CDK activity during meiosis, the regulation of CDK activity during meiosis, and mechanisms by which CDKs regulate proper meiotic chromosome segregation. First, a striking diversity in Clb-CDK activity is observed during meiosis, including the identification of Clb1-CDK, and Clb3-CDK as meiosis I and meiosis II specific Clb-CDKs respectively. Second, Clb3 protein is shown to be restricted to meiosis II by translational control mediated by the 5'UTR of the CLB3 message. Finally, premature production of Clb3 results in the premature separation of sister-chromatids during meiosis I.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Biology.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2010

Author dc:creator

• Carlile, Thomas M. (Thomas Marshal), Jr

Advisor dc:contributor.advisor

• Angelika Amon.

• Biology.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/57557

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/57557