{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/55127"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/55127","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Microfluidic in vivo laser microsurgery screen for identification of compounds enhancing neural regeneration","abstract":"Discovery of small molecules and novel mechanisms for enhancing neurite regeneration in animal models is significant for therapeutics of central nervous system injuries and neurodegenerative disorders. C. elegans is a widely studied model organisms due to their fully mapped neural network of 302 neurons and amenable genetics. Their small size and short life cycle allows for rapid studies to be conducted; however, after decades of use the manual methods of manipulation have still remained unchanged. This thesis details the development of automated, high-throughput optical and microfluidic technologies for screening C. elegans and demonstrates the production of a reliable system for screening over ten thousand animals. Using the screening system, femtosecond laser microsurgery was performed on thousands of animals followed by incubation in compounds from a chemical library. The screens revealed several high-scoring drug candidates that enhance regeneration after laser microsurgery of C. elegans mechanosensory neurons.","abstract_html":"Discovery of small molecules and novel mechanisms for enhancing neurite regeneration in animal models is significant for therapeutics of central nervous system injuries and neurodegenerative disorders. C. elegans is a widely studied model organisms due to their fully mapped neural network of 302 neurons and amenable genetics. Their small size and short life cycle allows for rapid studies to be conducted; however, after decades of use the manual methods of manipulation have still remained unchanged. This thesis details the development of automated, high-throughput optical and microfluidic technologies for screening C. elegans and demonstrates the production of a reliable system for screening over ten thousand animals. Using the screening system, femtosecond laser microsurgery was performed on thousands of animals followed by incubation in compounds from a chemical library. The screens revealed several high-scoring drug candidates that enhance regeneration after laser microsurgery of C. elegans mechanosensory neurons.","abstract_has_math":false,"creators":["Gilleland, Cody Lee"],"institution":"Massachusetts Institute of Technology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.","school":null,"contributors":[],"advisors":["Mehmet Fatih Yanik."],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009","date_published":"2009","updated_at":"2026-07-22T22:21:05Z","subjects":["Electrical Engineering and Computer Science."],"languages":["eng"],"rights":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 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The screens revealed several high-scoring drug candidates that enhance regeneration after laser microsurgery of C. elegans mechanosensory neurons."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["S.M."]},{"key":"dc:title","label":"Title","values":["Microfluidic in vivo laser microsurgery screen for identification of compounds enhancing neural regeneration"]}]}],"canonical_facts":{"dc:contributor.advisor":["Mehmet Fatih Yanik."],"dc:contributor.department":["Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science."],"dc:contributor.other":["Massachusetts Institute of Technology. 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This thesis details the development of automated, high-throughput optical and microfluidic technologies for screening C. elegans and demonstrates the production of a reliable system for screening over ten thousand animals. Using the screening system, femtosecond laser microsurgery was performed on thousands of animals followed by incubation in compounds from a chemical library. The screens revealed several high-scoring drug candidates that enhance regeneration after laser microsurgery of C. elegans mechanosensory neurons."],"dc:description.degree":["S.M."],"dc:identifier.uri":["http://hdl.handle.net/1721.1/55127"],"dc:language.iso":["eng"],"dc:publisher":["Massachusetts Institute of Technology"],"dc:rights":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 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