An examination of peptide-tether binding to gold and glass surfaces

For many biological applications, it is beneficial to know that a peptide will bind to a surface. In this thesis, a tether, bead, peptide complex is constructed using the gold binding peptide (GBP) of sequence (CGGVSGSSPDS). Several assays and assay modifications are developed and tested to attempt to attach the GBP first to a gold slide and then to gold nanoparticles. Four peptides: FO₂, K1, K2, K3, known to bind to sapphire were attached to glass to see if it was possible to modify the sapphire assay to work with glass.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Mechanical Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2009

Author dc:creator

• Kunkel, Jacquelyn Anne

Advisor dc:contributor.advisor

• Matthew J. Lang.

• Mechanical Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/54507

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/54507