{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/54228"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/54228","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Polyelectrolyte multilayers for tunable release of antibiotics and other therapeutics","abstract":"Polyelectrolyte multilayers (PEMs) were fabricated via the layer-by-layer (LbL) deposition process, incorporating hydrolytically degradable poly([beta]-amino esters) to result in biodegradable PEMs that can release active ingredients in a dosage- and rate-tunable fashion. Specifically, PEMs incorporating several types of antibiotics, ranging from aminoglycosides to antimicrobial peptides (AmPs), were fabricated and characterized; these coatings are intended for applications onto biomedical device surfaces for infection control. In vitro efficacy against Staphylococcus aureus and nontoxicity towards preosteoblasts MC3T3 were demonstrated. In vivo evaluations involving a rabbit osteomyelitis model were undertaken as well. Aside from the development of antimicrobial PEMs, additional projects pursued under this thesis, all in the context of PEM-based drug delivery, include [1] demonstration of the sequential release of two species, [2] assessment of the in vitro activity of anticoagulant films, [3] delivery of siRNAs, [4] evaluation of the biocompatibility of poly([beta]- amino esters), [5] incorporation of cyclodextrins for the purpose of small molecule delivery, [6] incorporation of poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating gentamicin, [7] evaluation of film sterilizability via FDA-approved methods, and [8] design and characterization of a multi-drug coating for orthopedic implants for dual antimicrobial and tissue regenerative actions.","abstract_html":"Polyelectrolyte multilayers (PEMs) were fabricated via the layer-by-layer (LbL) deposition process, incorporating hydrolytically degradable poly([beta]-amino esters) to result in biodegradable PEMs that can release active ingredients in a dosage- and rate-tunable fashion. Specifically, PEMs incorporating several types of antibiotics, ranging from aminoglycosides to antimicrobial peptides (AmPs), were fabricated and characterized; these coatings are intended for applications onto biomedical device surfaces for infection control. In vitro efficacy against Staphylococcus aureus and nontoxicity towards preosteoblasts MC3T3 were demonstrated. In vivo evaluations involving a rabbit osteomyelitis model were undertaken as well. Aside from the development of antimicrobial PEMs, additional projects pursued under this thesis, all in the context of PEM-based drug delivery, include [1] demonstration of the sequential release of two species, [2] assessment of the in vitro activity of anticoagulant films, [3] delivery of siRNAs, [4] evaluation of the biocompatibility of poly([beta]- amino esters), [5] incorporation of cyclodextrins for the purpose of small molecule delivery, [6] incorporation of poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating gentamicin, [7] evaluation of film sterilizability via FDA-approved methods, and [8] design and characterization of a multi-drug coating for orthopedic implants for dual antimicrobial and tissue regenerative actions.","abstract_has_math":false,"creators":["Chuang, Helen F"],"institution":"Massachusetts Institute of Technology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. 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Aside from the development of antimicrobial PEMs, additional projects pursued under this thesis, all in the context of PEM-based drug delivery, include [1] demonstration of the sequential release of two species, [2] assessment of the in vitro activity of anticoagulant films, [3] delivery of siRNAs, [4] evaluation of the biocompatibility of poly([beta]- amino esters), [5] incorporation of cyclodextrins for the purpose of small molecule delivery, [6] incorporation of poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating gentamicin, [7] evaluation of film sterilizability via FDA-approved methods, and [8] design and characterization of a multi-drug coating for orthopedic implants for dual antimicrobial and tissue regenerative actions."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph.D."]},{"key":"dc:title","label":"Title","values":["Polyelectrolyte multilayers for tunable release of antibiotics and other therapeutics"]}]}],"canonical_facts":{"dc:contributor.advisor":["Paula T. 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