Development of an integrated capillary valve-based preconcentrator and surface-based immunoassay

A new generation of integrated preconcentrator and immunoassay was developed. A novel, self-aligned method for patterning Nafion resin was developed and applied to create a preconcentrator. In a parallel effort, a surface-based immunoassay was developed and optimized for use with the preconcentrator. Experiments were carried out with R-phycoerythrin (R-PE) to characterize the performance of the integrated preconcentrator and immunoassay. The immunoassay originally has a detection limit of Ing/mL of R-PE in 1xPBS. When coupled to the preconcentrator, this limit has been improved to 0.Olng/mL, a roughly 100x improvement. Discussions of the differences between this and previous generations of concentrators are also presented in this thesis.

Department dc:contributor.department

Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2009

Author dc:creator

• Liu, Vincent Hok

Advisor dc:contributor.advisor

• Jongyoon Han.

• Electrical Engineering and Computer Science.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/47815

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/47815