{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/45309"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/45309","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"Investigations into the role of protein phosphatases in the EGFR signaling network","abstract":"Protein phosphatases regulate the phosphorylation state of intracellular signaling molecules in conjunction with protein kinases. In order to better understand the role of phosphatases in the ErbB signaling network, experimental studies were carried out to validate assays and phosphatase inhibitors for gathering dynamic, system-wide data of phosphatase activity. We verified the use of In-Cell Westerns and phospho-ErbB ELISAs for these studies, as well as the use of different cell lines and both general and specific phosphatase inhibitors in system-wide experiments. The phosphatase inhibitors we used perturbed cellular systems in complex ways that can help elucidate the regulation and activity of specific phosphatases in the ErbB signaling pathway in the future.","abstract_html":"Protein phosphatases regulate the phosphorylation state of intracellular signaling molecules in conjunction with protein kinases. In order to better understand the role of phosphatases in the ErbB signaling network, experimental studies were carried out to validate assays and phosphatase inhibitors for gathering dynamic, system-wide data of phosphatase activity. We verified the use of In-Cell Westerns and phospho-ErbB ELISAs for these studies, as well as the use of different cell lines and both general and specific phosphatase inhibitors in system-wide experiments. The phosphatase inhibitors we used perturbed cellular systems in complex ways that can help elucidate the regulation and activity of specific phosphatases in the ErbB signaling pathway in the future.","abstract_has_math":false,"creators":["Lu, Kuojung Gordon"],"institution":"Massachusetts Institute of Technology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Dept. of Biology.","school":null,"contributors":[],"advisors":["Peter K. Sorger."],"committee_chairs":[],"committee_members":[],"year":2008,"date_issued":"2008","date_published":"2008","updated_at":"2026-07-22T22:22:08Z","subjects":["Biology."],"languages":["eng"],"rights":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission."],"rights_urls":["http://dspace.mit.edu/handle/1721.1/7582"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1721.1/45309","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Peter K. Sorger."]},{"key":"dc:contributor.department","label":"Department","values":["Massachusetts Institute of Technology. Dept. of Biology."]},{"key":"dc:contributor.other","label":"Dc Contributor Other","values":["Massachusetts Institute of Technology. Dept. of Biology."]},{"key":"dc:creator","label":"Author","values":["Lu, Kuojung Gordon"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2009-04-29T17:23:44Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2009-04-29T17:23:44Z"]},{"key":"dc:date.issued","label":"Date","values":["2008"]},{"key":"dc:publisher","label":"Institution","values":["Massachusetts Institute of Technology"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission."]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://dspace.mit.edu/handle/1721.1/7582"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1721.1/45309"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Biology, 2008.","Includes bibliographical references (leaves 39-43)."]},{"key":"dc:description.abstract","label":"Abstract","values":["Protein phosphatases regulate the phosphorylation state of intracellular signaling molecules in conjunction with protein kinases. In order to better understand the role of phosphatases in the ErbB signaling network, experimental studies were carried out to validate assays and phosphatase inhibitors for gathering dynamic, system-wide data of phosphatase activity. We verified the use of In-Cell Westerns and phospho-ErbB ELISAs for these studies, as well as the use of different cell lines and both general and specific phosphatase inhibitors in system-wide experiments. The phosphatase inhibitors we used perturbed cellular systems in complex ways that can help elucidate the regulation and activity of specific phosphatases in the ErbB signaling pathway in the future."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["S.M."]},{"key":"dc:title","label":"Title","values":["Investigations into the role of protein phosphatases in the EGFR signaling network"]}]}],"canonical_facts":{"dc:contributor.advisor":["Peter K. Sorger."],"dc:contributor.department":["Massachusetts Institute of Technology. Dept. of Biology."],"dc:contributor.other":["Massachusetts Institute of Technology. Dept. of Biology."],"dc:creator":["Lu, Kuojung Gordon"],"dc:date.accessioned":["2009-04-29T17:23:44Z"],"dc:date.available":["2009-04-29T17:23:44Z"],"dc:date.issued":["2008"],"dc:description":["Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Biology, 2008.","Includes bibliographical references (leaves 39-43)."],"dc:description.abstract":["Protein phosphatases regulate the phosphorylation state of intracellular signaling molecules in conjunction with protein kinases. In order to better understand the role of phosphatases in the ErbB signaling network, experimental studies were carried out to validate assays and phosphatase inhibitors for gathering dynamic, system-wide data of phosphatase activity. We verified the use of In-Cell Westerns and phospho-ErbB ELISAs for these studies, as well as the use of different cell lines and both general and specific phosphatase inhibitors in system-wide experiments. The phosphatase inhibitors we used perturbed cellular systems in complex ways that can help elucidate the regulation and activity of specific phosphatases in the ErbB signaling pathway in the future."],"dc:description.degree":["S.M."],"dc:identifier.uri":["http://hdl.handle.net/1721.1/45309"],"dc:language.iso":["eng"],"dc:publisher":["Massachusetts Institute of Technology"],"dc:rights":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission."],"dc:rights.uri":["http://dspace.mit.edu/handle/1721.1/7582"],"dc:subject":["Biology."],"dc:title":["Investigations into the role of protein phosphatases in the EGFR signaling network"],"dc:type":["Thesis"]},"updated_at":"2026-07-22T22:22:08Z"}