{"id":{"repo_id":"mit","oai_identifier":"oai:dspace.mit.edu:1721.1/44423"},"canonical_url":"https://search.dev.ndltd.org/etd/mit/oai:dspace.mit.edu:1721.1/44423","repository":{"repo_id":"mit","name":"MIT","base_url":"https://dspace.mit.edu/oai/request"},"display":{"title":"DNA as a programmable material : de novo gene synthesis and error correction","abstract":"Deoxyribonucleic acid (DNA), the polymeric molecule that carries the genetic code of all living organisms, is arguably one of the most programmable assembly materials available to chemists, biologists, and materials scientists. Scientists have used DNA to build many different structures for various applications in disparate areas of research from traditional biological applications to more recent non-biological applications. Although DNA isn't typically thought of as an assembly material by people not doing research in the area, the availability of decreasing cost synthetic oligonucleotides has led to advances in gene fabrication technology which in turn has enabled synthetic biology to flourish. Using DNA as a building material for small and large constructs of DNA is reliant on having effective gene synthesis techniques. Construction of synthetic DNA is limited by errors that pervade the final product. To address this problem, effective error correction methods are pivotal. Having extremely robust gene synthesis and error correction techniques will allow researchers to generate very large scale constructs potentially necessary in applications such as genome re-engineering.","abstract_html":"Deoxyribonucleic acid (DNA), the polymeric molecule that carries the genetic code of all living organisms, is arguably one of the most programmable assembly materials available to chemists, biologists, and materials scientists. Scientists have used DNA to build many different structures for various applications in disparate areas of research from traditional biological applications to more recent non-biological applications. Although DNA isn't typically thought of as an assembly material by people not doing research in the area, the availability of decreasing cost synthetic oligonucleotides has led to advances in gene fabrication technology which in turn has enabled synthetic biology to flourish. Using DNA as a building material for small and large constructs of DNA is reliant on having effective gene synthesis techniques. Construction of synthetic DNA is limited by errors that pervade the final product. To address this problem, effective error correction methods are pivotal. Having extremely robust gene synthesis and error correction techniques will allow researchers to generate very large scale constructs potentially necessary in applications such as genome re-engineering.","abstract_has_math":false,"creators":["Hwang, Samuel James"],"institution":"Massachusetts Institute of Technology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Massachusetts Institute of Technology. Dept. of Materials Science and Engineering.","school":null,"contributors":[],"advisors":["Joseph Jacobson."],"committee_chairs":[],"committee_members":[],"year":2008,"date_issued":"2008","date_published":"2008","updated_at":"2026-07-22T22:20:57Z","subjects":["Materials Science and Engineering."],"languages":["eng"],"rights":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission."],"rights_urls":["http://dspace.mit.edu/handle/1721.1/7582"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1721.1/44423","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Joseph Jacobson."]},{"key":"dc:contributor.department","label":"Department","values":["Massachusetts Institute of Technology. Dept. of Materials Science and Engineering."]},{"key":"dc:contributor.other","label":"Dc Contributor Other","values":["Massachusetts Institute of Technology. Dept. of Materials Science and Engineering."]},{"key":"dc:creator","label":"Author","values":["Hwang, Samuel James"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2009-01-30T16:45:02Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2009-01-30T16:45:02Z"]},{"key":"dc:date.issued","label":"Date","values":["2008"]},{"key":"dc:publisher","label":"Institution","values":["Massachusetts Institute of Technology"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Materials Science and Engineering."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission."]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://dspace.mit.edu/handle/1721.1/7582"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1721.1/44423"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2008.","Includes bibliographical references (leaves 42-43)."]},{"key":"dc:description.abstract","label":"Abstract","values":["Deoxyribonucleic acid (DNA), the polymeric molecule that carries the genetic code of all living organisms, is arguably one of the most programmable assembly materials available to chemists, biologists, and materials scientists. Scientists have used DNA to build many different structures for various applications in disparate areas of research from traditional biological applications to more recent non-biological applications. Although DNA isn't typically thought of as an assembly material by people not doing research in the area, the availability of decreasing cost synthetic oligonucleotides has led to advances in gene fabrication technology which in turn has enabled synthetic biology to flourish. Using DNA as a building material for small and large constructs of DNA is reliant on having effective gene synthesis techniques. Construction of synthetic DNA is limited by errors that pervade the final product. To address this problem, effective error correction methods are pivotal. Having extremely robust gene synthesis and error correction techniques will allow researchers to generate very large scale constructs potentially necessary in applications such as genome re-engineering."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["S.M."]},{"key":"dc:title","label":"Title","values":["DNA as a programmable material : de novo gene synthesis and error correction"]}]}],"canonical_facts":{"dc:contributor.advisor":["Joseph Jacobson."],"dc:contributor.department":["Massachusetts Institute of Technology. Dept. of Materials Science and Engineering."],"dc:contributor.other":["Massachusetts Institute of Technology. Dept. of Materials Science and Engineering."],"dc:creator":["Hwang, Samuel James"],"dc:date.accessioned":["2009-01-30T16:45:02Z"],"dc:date.available":["2009-01-30T16:45:02Z"],"dc:date.issued":["2008"],"dc:description":["Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2008.","Includes bibliographical references (leaves 42-43)."],"dc:description.abstract":["Deoxyribonucleic acid (DNA), the polymeric molecule that carries the genetic code of all living organisms, is arguably one of the most programmable assembly materials available to chemists, biologists, and materials scientists. Scientists have used DNA to build many different structures for various applications in disparate areas of research from traditional biological applications to more recent non-biological applications. Although DNA isn't typically thought of as an assembly material by people not doing research in the area, the availability of decreasing cost synthetic oligonucleotides has led to advances in gene fabrication technology which in turn has enabled synthetic biology to flourish. Using DNA as a building material for small and large constructs of DNA is reliant on having effective gene synthesis techniques. Construction of synthetic DNA is limited by errors that pervade the final product. To address this problem, effective error correction methods are pivotal. Having extremely robust gene synthesis and error correction techniques will allow researchers to generate very large scale constructs potentially necessary in applications such as genome re-engineering."],"dc:description.degree":["S.M."],"dc:identifier.uri":["http://hdl.handle.net/1721.1/44423"],"dc:language.iso":["eng"],"dc:publisher":["Massachusetts Institute of Technology"],"dc:rights":["M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission."],"dc:rights.uri":["http://dspace.mit.edu/handle/1721.1/7582"],"dc:subject":["Materials Science and Engineering."],"dc:title":["DNA as a programmable material : de novo gene synthesis and error correction"],"dc:type":["Thesis"]},"updated_at":"2026-07-22T22:20:57Z"}